[Histonet] H&E Troubleshooting

[Teri Johnson]

Hi Cassie,

I would strongly suspect your tap water. If you have a tap water rinse before hematoxylin, the pH or treatment additives carrying over could be weakening your stain. We fixed this problem previously by adding a bucket of DI water between the tap water wash and the hematoxylin, and that fixed it for us.

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohnson at genoptix.com<mailto:tejohnson at genoptix.com>
www.genoptix.com<http://www.genoptix.com/>


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