[Histonet] Collodian bag for cell blocks

[Julio Benavides]

Hi,

I have a question regarding the use of UCSF Collodion Bag Cell blocks.  
once you do the bag, you pour in the cells in, let´s say 10ml of  
formalin. Then, how do you proceed? Do you spin down the cells with  
the bag inside a plastic tube? do you take then put the formalin and  
cut the top of the bag into a cassette? do you close the bag with a  
thread with the cells and formalin?....

Any help with this would be greatly appreciated,

Thanks a lot

julio



WILLIAM DESALVO via Histonet <histonet at lists.utsouthwestern.edu> escribió:

> What method are you using? Making your own coated tubes, UCSF or MD  
> Anderson. Are you purchasing coated tubes? Once the tube is coated,  
> dried (using hood) and filled w dH2O, there should be no odor.  
> Always work w/ collodion under the hood. Ethyl ether is main  
> component. Once in cassette and processed, there should not be odor.
>
> Sent from my Windows Phone
> ________________________________
> From: Baldwin, Kathy via Histonet<mailto:histonet at lists.utsouthwestern.edu>
> Sent: ‎1/‎4/‎2016 2:07 PM
> To:  
> 'histonet at lists.utsouthwestern.edu'<mailto:histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Collodian bag for cell blocks
>
> Hi All
> Was wondering if anyone is using the Collodian bag for cell blocks.   
> We just got it and have been using it 'works great' but all the  
> techs are complaining of the smell.. Our ventilation has been looked  
> at and has passed however the smell still lingers in the room.  Any  
> suggestions??
>
>
> Thanks
> S. Kathy Baldwin
> Histology/Cytology Supervisor
> Memorial Hospital and Health Care Center
> 800 West 9th St.
> Jasper, Indiana  47546
> Office 812-996-0210
> Fax 812-996-0232
> Cell 812-887-3357
>
>
>
>
>
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