[Histonet] formalin and shrinkage
gu.lang at gmx.at
Mon Feb 22 14:16:40 CST 2016
My question refers to the difference in dimensions of the native tissue and the fixed tissue. – so without any influence of ethanol and xylol.
I have no access to the whole publication you mentioned. If I understand the summary correctly, 2,5% shrinkage was found after formalinfixation.
There is no actual problem to solve, just academic question.
Von: Jay Lundgren [mailto:jaylundgren at gmail.com]
Gesendet: Montag, 22. Februar 2016 20:31
An: gu.lang at gmx.at
Betreff: Re: [Histonet] formalin and shrinkage
I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I guess that's 30% shrinkage overall? Shrinkage is partially caused by formalin crosslinking the proteins in fixation, and partially by dehydration. Maybe a little shrinkage in xylene too? From removal of fat in adipose tissues?
Is your Pathologist really concerned about shrinkage, or about curling and distortion of small shave bxs? Because a certain degree of shrinkage is an unavoidable artifact of tissue processing.
If it's the latter, I like to use 2 blue sponges. I find they really help to keep things flat and oriented. Some people don't like them because of carryover. I just say change your processor reagents more often.
Jay A. Lundgren, M.S., HTL (ASCP)
On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu> wrote:
Today someone asked me about shrinkage caused by the fixation with
formaldehyde specially on skin-biopsies. She spoke about shrinkage of 30%
percent. In my opinion shrinkage is mainly caused by the processing with
dehydration and defatting. Formaldehyde renders the tissue harder but not
What is the opinion of the community?
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