[Histonet] formalin and shrinkage

[Michael Ann Jones]

Agree with you Jay Lundgren.

Michael Ann Jones, HT (ASCP)
Histology Manager
Metropath
7444 W. Alaska Dr. #250
Lakewood, CO 80226
303.634.2511
Mjones at metropath.com






On 2/22/16, 12:30 PM, "Jay Lundgren via Histonet"
<histonet at lists.utsouthwestern.edu> wrote:

>I was taught at AFIP to expect shrinkage of 10%, in each dimension.  So I
>guess that's 30% shrinkage overall?  Shrinkage is partially caused by
>formalin crosslinking the proteins in fixation, and partially by
>dehydration.  Maybe a little shrinkage in xylene too?  From removal of fat
>in adipose tissues?
>http://link.springer.com/article/10.1007/BF00695061#page-1
>
>Is your Pathologist really concerned about shrinkage, or about curling and
>distortion of small shave bxs?  Because a certain degree of shrinkage is
>an
>unavoidable artifact of tissue processing.
>
>If it's the latter, I like to use 2 blue sponges.  I find they really help
>to keep things flat and oriented.  Some people don't like them because of
>carryover.  I just say change your processor reagents more often.
>
>Sincerely,
>
>    Jay A. Lundgren, M.S., HTL (ASCP)
>
>
>On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet <
>histonet at lists.utsouthwestern.edu> wrote:
>
>> Hi!
>>
>> Today someone asked me about shrinkage caused by the fixation with
>> formaldehyde specially on skin-biopsies.  She spoke about shrinkage of
>>30%
>> percent. In my opinion shrinkage is mainly caused by the processing with
>> dehydration and defatting. Formaldehyde renders the tissue harder but
>>not
>> strictly smaller.
>>
>>
>>
>> What is the opinion of the community?
>>
>>
>>
>> Gudrun
>>
>>
>>
>>
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>_______________________________________________
>Histonet mailing list
>Histonet at lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet