[Histonet] formalin and shrinkage
Michael Ann Jones
mjones at metropath.com
Mon Feb 22 13:48:20 CST 2016
Agree with you Jay Lundgren.
Michael Ann Jones, HT (ASCP)
7444 W. Alaska Dr. #250
Lakewood, CO 80226
Mjones at metropath.com
On 2/22/16, 12:30 PM, "Jay Lundgren via Histonet"
<histonet at lists.utsouthwestern.edu> wrote:
>I was taught at AFIP to expect shrinkage of 10%, in each dimension. So I
>guess that's 30% shrinkage overall? Shrinkage is partially caused by
>formalin crosslinking the proteins in fixation, and partially by
>dehydration. Maybe a little shrinkage in xylene too? From removal of fat
>in adipose tissues?
>Is your Pathologist really concerned about shrinkage, or about curling and
>distortion of small shave bxs? Because a certain degree of shrinkage is
>unavoidable artifact of tissue processing.
>If it's the latter, I like to use 2 blue sponges. I find they really help
>to keep things flat and oriented. Some people don't like them because of
>carryover. I just say change your processor reagents more often.
> Jay A. Lundgren, M.S., HTL (ASCP)
>On Mon, Feb 22, 2016 at 9:59 AM, Gudrun Lang via Histonet <
>histonet at lists.utsouthwestern.edu> wrote:
>> Today someone asked me about shrinkage caused by the fixation with
>> formaldehyde specially on skin-biopsies. She spoke about shrinkage of
>> percent. In my opinion shrinkage is mainly caused by the processing with
>> dehydration and defatting. Formaldehyde renders the tissue harder but
>> strictly smaller.
>> What is the opinion of the community?
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
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