[Histonet] question - Allergy to histological solvents?

Rene J Buesa rjbuesa at yahoo.com
Sun Feb 21 09:29:09 CST 2016

You can dewax absolutely safely using a 2% dishawasher soap solution at 90ºC (twice) as washing in water.You can "dehydrate" stained stains by placing the slides in an oven at 60ºC, also absolutely safely for the stained section.Under separate cover I am sending  articles on this subject.René 

    On Saturday, February 20, 2016 3:05 PM, daniel blackburn via Histonet <histonet at lists.utsouthwestern.edu> wrote:


After decades of using standard organic solvents for paraffin- section histology, I find that I've become highly allergic to the fumes.  These include commercial preps like Citrosolv and Hemo-De, as well as xylene and toluene, which cause (in me) dizziness and a pounding headache.  This problem has made staining and coverslipping very difficult, even with  hood.  Can anyone recommend alternative solutions for (a) dewaxing prior to staining and (b) coverslipping?  thanks very much!

Daniel Blackburn
Biology, Trinity College (Hartford CT)

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