[Histonet] clearing time

[Elizabeth Chlipala]

Mohamed

For larger samples we have ranges from 1.5  hours to 6 hours a station.  Anytime we have larger samples we always utilize three absolute alcohols and three xylenes, for your samples I would try 1.5 to 2 hours per station to start.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

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Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: mohamed abd el razik via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, February 04, 2016 11:52 AM
To: Histonet at lists.utsouthwestern.edu
Subject: [Histonet] clearing time

Dear all 
what is your optimal clearing time in xelene for prostate and tests of dog samples (about 0.5 cm) ??is there a need for additional clearing in methyl benzoat ?


thanks 
Mohamed

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