[Histonet] Staining issues

Rene J Buesa rjbuesa at yahoo.com
Wed Dec 28 08:09:42 CST 2016

Improper staining at the center and falling sections are typical consequences of poor fixation/infiltration.If you have changed nothing proceduraly, what about somebody "new" grossing and preparing thicker tissue slices?René 

    On Wednesday, December 28, 2016 8:34 AM, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:

 Our pathologists are complaining that the center of tissues are not
staining properly in both our H&E's and IHC's. Can anyone provide some
thoughts as to where this problem could be occurring?

As a separate situation we are experiencing the tissues folding or falling
off during the staining process. Both this issue and the staining issue are
recent occurrences and we have not changed our process in any way from
previous years.

Does anyone think these problems are related? Why or why not?  I have
racked my brain with all the trouble shooting techniques I can think of so
any help will greatly be appreciated


Charles Riley HT(ASCP)CM

Histopathology Coordinator/ Mohs
Histonet mailing list
Histonet at lists.utsouthwestern.edu


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