[Histonet] storage big samples

[Blanca Lopez]

Hello!
I was wondering if anybody has an idea how can to storage the whole prostate tissue in -80c. What kind of container we can buy or media or bags, or anything to keep it safe.. Also, I need a good advice in how to cut frozen and re-storage in -80 C. How can I removed the oct without expose the tissue too much. The first time I thaw the tissue in water to remove the oct because is to big that is really hard to place it on my mold. I was able to cut frozen slides but the procedure that they are doing did not work. Do I am, doing something wrong? I really need a good advice in handling cutting frozen, stain them and storage them back in -80c.
Any suggestions that can help me?
thanks

Blanca Lopez
Histotech (ASCP)
UTSW Tissue Resource K1.210
Simmons Comprehensive Cancer Center
UT Southwestern Medical Center
Telephone: 214-648-7598
Email: Blanca.Lopez at utsouthwestern.edu


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