[Histonet] ER/PR Uneven Staining

Gagnon, Eric gagnone at KGH.KARI.NET
Mon Dec 5 13:59:52 CST 2016

As Greg has already  mentioned, placing control sections on each patient slide will assist in troubleshooting your staining issue. Joanne, I don't believe you mentioned it in your initial email, but did you attempt a repeat and if so, did the results replicate on the repeat? Occasionally there may be a mixing issue or other physical issue with the covertile that inhibits complete reaction across the slide.

Eric Gagnon MLT
Histology Laboratory
Kingston General Hospital,
Kingston, Ontario, Canada

From: Joanne Clark via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Tuesday, November 29, 2016 4:09 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] ER/PR Uneven Staining

I had a breast needle core today that when stained with ER and PR the staining was uneven throughout the core, even though the cancer cells were present in the entire core.  The specimen had 10 hours fixation in 10% NBF.  I could understand the uneven staining from inadequate fixation on large grossed in breast tissue, but 10 hours with  needle core biopsies has always been more than sufficient.  Does anyone have any ideas?  We use Leica's ER and PR antibodies on the BOND.

Joanne Clark, BAAS, HT(ASCP)CM
Director of Histology

P.   (575) 622-5600
C.   (575) 317-6403
F.   (575) 622-3720
TF. (800) 753-7284


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