[Histonet] Histonet Digest, Vol 153, Issue 16
Corey Hubbard
corey at rankinbiomed.com
Fri Aug 19 12:13:09 CDT 2016
Hi Jim/Histonet world,
This was a mistake and was not supposed to be posted. Histonet has already
been emailed. Thank you! :)
Message: 1
Date: Thu, 18 Aug 2016 13:23:34 -0400
From: Jim Anzaldi <janzaldi at medequipsource.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 15
Message-ID:
<2C7597ABF5467247BE159D763E27F839016714B99787 at PROMETHEUS.
medequip.local>
Content-Type: text/plain; charset="us-ascii"
Vendor is soliciting sales item #1
*Corey Hubbard *
Sales Account Manager / Marketing Admin
248.215.5390 ext 0022
*RANKIN*
14515 Mackey Road
Holly, MI 48442
www.rankinbiomed.com
facebook.com/rankinbiomed
twitter.com/rankinbiomed.com
*John 8:12* Jesus spoke to them, saying "I am the light of the world; he
who follows me will not walk in darkness, but will have the light of life.
On Fri, Aug 19, 2016 at 1:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:
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> Today's Topics:
>
> 1. Re: Histonet Digest, Vol 153, Issue 15 (Jim Anzaldi)
> 2. Re: Gallocyanin (Bob Richmond)
> 3. Re: Gallocyanin - protocol (Elizabeth Chlipala)
> 4. Re: Gallocyanine (Bob Richmond)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 18 Aug 2016 13:23:34 -0400
> From: Jim Anzaldi <janzaldi at medequipsource.com>
> To: "histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 15
> Message-ID:
> <2C7597ABF5467247BE159D763E27F839016714B99787 at PROMETHEUS.
> medequip.local>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Vendor is soliciting sales item #1
>
> -----Original Message-----
> From: histonet-request at lists.utsouthwestern.edu [mailto:histonet-request@
> lists.utsouthwestern.edu]
> Sent: Thursday, August 18, 2016 1:00 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 153, Issue 15
>
> Send Histonet mailing list submissions to
> histonet at lists.utsouthwestern.edu
>
> To subscribe or unsubscribe via the World Wide Web, visit
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
> histonet-request at lists.utsouthwestern.edu
>
> You can reach the person managing the list at
> histonet-owner at lists.utsouthwestern.edu
>
> When replying, please edit your Subject line so it is more specific than
> "Re: Contents of Histonet digest..."
>
>
> Today's Topics:
>
> 1. update (Corey Hubbard)
> 2. Re: Gallocyanine (Bob Richmond)
> 3. Re: Gallocyanine (Elizabeth Chlipala)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 17 Aug 2016 14:15:29 -0400
> From: Corey Hubbard <corey at rankinbiomed.com>
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] update
> Message-ID:
> <CAKSgtkf81Hv=jwsnFJqmYqwOmJe515QZ1uBq3RdcQBs6V+fS3g at mail.
> gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Hi Eva,
>
> I wanted to see how things are going for you. Please let me know if you
> come across any equipment needs. We specialize in histology and pathology
> equipment and service.
>
> We are also always looking to purchase equipment - broken, used,
> collecting dust. etc.
>
> Do you have a vendor you use currently for equipment?
>
>
>
> *Corey Hubbard *
> Sales Account Manager / Marketing Admin
> 248.215.5390 ext 0022
>
> *RANKIN*
> 14515 Mackey Road
> Holly, MI 48442
> www.rankinbiomed.com
> facebook.com/rankinbiomed
> twitter.com/rankinbiomed.com
>
> *John 8:12* Jesus spoke to them, saying "I am the light of the world; he
> who follows me will not walk in darkness, but will have the light of life.
>
>
> ------------------------------
>
> Message: 2
> Date: Wed, 17 Aug 2016 19:58:38 -0400
> From: Bob Richmond <rsrichmond at gmail.com>
> To: "Histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Gallocyanine
> Message-ID:
> <CAOKsRH6Mi0ff3xC3NqgVifbTrUPhfM0EaAOxRcE0okhEvPF=6Q at mail.
> gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Eva Permaul at Georgetown University asks:
>
> >>We just had a request for Gallocyanine staining. Does anyone do this?
> >>Can
> you share your protocol? Where do you buy your reagents?<<
>
> Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat
> similar to celestin blue B. It's been used as a stain for Nissl substance.
> Somebody at the AFIP was touting it as a hematoxylin substitute during the
> Great Hematoxylin Shortage of around 1972. I think it was used as a chrome
> alum lake for a nuclear stain. I've never seen it. You can get the dry dye
> from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both
> have formulas. But what does your client want to do with it?
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
>
> ------------------------------
>
> Message: 3
> Date: Thu, 18 Aug 2016 08:03:45 -0600
> From: Elizabeth Chlipala <liz at premierlab.com>
> To: Bob Richmond <rsrichmond at gmail.com>,
> "'histonet at lists.utsouthwestern.edu'
> (histonet at lists.utsouthwestern.edu)"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Gallocyanine
> Message-ID:
> <14E2C6176416974295479C64A11CB9AE02BED535B69A at SBS2K8.
> premierlab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We have run this stain before, purchased the reagent from Sigma, it's
> pretty easy to do there is a procedure in Bancroft and Gamble.
>
> Liz
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Premier Laboratory, LLC PO Box
> 18592 Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz at premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -----Original Message-----
> From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Wednesday, August 17, 2016 5:59 PM
> To: Histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Gallocyanine
>
> Eva Permaul at Georgetown University asks:
>
> >>We just had a request for Gallocyanine staining. Does anyone do this?
> >>Can
> you share your protocol? Where do you buy your reagents?<<
>
> Gallocyanin (correct spelling - C.I. 51030) is a blue oxazine dye somewhat
> similar to celestin blue B. It's been used as a stain for Nissl substance.
> Somebody at the AFIP was touting it as a hematoxylin substitute during the
> Great Hematoxylin Shortage of around 1972. I think it was used as a chrome
> alum lake for a nuclear stain. I've never seen it. You can get the dry dye
> from Sigma-Aldrich. John Kiernan's book and Bancroft & Stevens 4th ed both
> have formulas. But what does your client want to do with it?
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Subject: Digest Footer
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> End of Histonet Digest, Vol 153, Issue 15
> *****************************************
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 18 Aug 2016 20:01:51 -0400
> From: Bob Richmond <rsrichmond at gmail.com>
> To: "Histonet at lists.utsouthwestern.edu"
> <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Gallocyanin
> Message-ID:
> <CAOKsRH7SOy458R_9CSM0XtKyKre_o4ayMLsANXW8HnEaQ3wDHw at mail.
> gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Liz Chlipala at Premier Laboratory in Boulder CO writes:
>
> >>We have run this stain [gallocyanin] before, purchased the reagent from
> Sigma, it's pretty easy to do there is a procedure in Bancroft and
> Gamble.<<
>
> What stain? What mordant (chrome, aluminum, none)? To stain what?
>
> I think the person originally posting needs to extract this information
> from the person who wants the stain done.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 19 Aug 2016 08:24:06 -0600
> From: Elizabeth Chlipala <liz at premierlab.com>
> To: Bob Richmond <rsrichmond at gmail.com>,
> "'histonet at lists.utsouthwestern.edu'
> (histonet at lists.utsouthwestern.edu)"
> <histonet at lists.utsouthwestern.edu>, "eca9 at georgetown.edu"
> <eca9 at georgetown.edu>
> Subject: Re: [Histonet] Gallocyanin - protocol
> Message-ID:
> <14E2C6176416974295479C64A11CB9AE02BED535B6BD at SBS2K8.
> premierlab.local>
> Content-Type: text/plain; charset="us-ascii"
>
> We used the stain to stain nissl substance in mouse lumbar spine.
>
> Purchased the dye from sigma - below is from the SDS Sheet
>
> Product name : Gallocyanine
> Product Number : 124508
> Brand : Sigma-Aldrich
> CAS-No. : 1562-85-2
>
> The procedure in Bancroft-Gamble uses Chrom Alum - which is chromium
> potassium sulfate as the mordant
>
> Chrom Alum - 5 gm
> dH20 - 100 ml
> Gallocyanine - 50 mg
>
> The chrome alum is dissolved in the distilled water, the gallocyanine is
> added and the solution slowly heated until it boils. Boil for 5 minutes.
> Cool to room temp adjust the volume back to 100 ml. Filter before use
>
> The stain is easy - place in staining solution for 18-48 hours, rinse in
> water, dehydrate, clear and mount. B&G states results are blue but If I
> remember correctly it turned out to be a bluish grey in color. I tried to
> see if I had any images but I could not find any, we did this back in 2006
> so it was some time ago.
>
> If you search the histonet archives there is an older post of mine looking
> for some help on this stain, there is a nice response back from Dr. Kiernan
> explaining a bit more about the dye and staining.
>
> Liz
>
>
> Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
> Premier Laboratory, LLC
> PO Box 18592
> Boulder, CO 80308
> (303) 682-3949 office
> (303) 682-9060 fax
> (303) 881-0763 cell
> liz at premierlab.com
> www.premierlab.com
>
> Ship to Address:
>
> Premier Laboratory, LLC
> 1567 Skyway Drive, Unit E
> Longmont, CO 80504
>
> -----Original Message-----
> From: Bob Richmond via Histonet [mailto:histonet at lists.utsouthwestern.edu]
> Sent: Thursday, August 18, 2016 6:02 PM
> To: Histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] Gallocyanin
>
> Liz Chlipala at Premier Laboratory in Boulder CO writes:
>
> >>We have run this stain [gallocyanin] before, purchased the reagent
> >>from
> Sigma, it's pretty easy to do there is a procedure in Bancroft and
> Gamble.<<
>
> What stain? What mordant (chrome, aluminum, none)? To stain what?
>
> I think the person originally posting needs to extract this information
> from the person who wants the stain done.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 19 Aug 2016 10:38:59 -0400
> From: Bob Richmond <rsrichmond at gmail.com>
> To: Elizabeth Chlipala <liz at premierlab.com>
> Cc: "'histonet at lists.utsouthwestern.edu'
> (histonet at lists.utsouthwestern.edu)"
> <histonet at lists.utsouthwestern.edu>, "eca9 at georgetown.edu"
> <eca9 at georgetown.edu>
> Subject: Re: [Histonet] Gallocyanine
> Message-ID:
> <CAOKsRH58yk3hYg6826HVJMco70tH8gyJgHj2yvgxsU-+Adwvzw at mail.
> gmail.com>
> Content-Type: text/plain; charset=UTF-8
>
> Here's John Kiernan's extremely informative review of gallocyanine (that's
> how he says it's to be spelled) from the archives - I can't find a date on
> it. Really tells you everything you need to know to do Nissl staining with
> it.
>
> http://www.histosearch.com/histonet/May06/Re.Histonetgallocyaninest.html
>
> Remember to check on hazardous waste issues for chromium.
>
> Bob Richmond
> Samurai Pathologist
> Maryville TN
>
>
> ------------------------------
>
> Subject: Digest Footer
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ------------------------------
>
> End of Histonet Digest, Vol 153, Issue 16
> *****************************************
>
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