[Histonet] Antibodies storage tips

[Elizabeth Chlipala]

We use freezer boxes and then record what is in the freezer boxes on a worksheet that is kept in an excel file.  The excel file has all of the boxes in it.



Here is an example of one of the pages


BOX 6 - Full





name

vendor

catalog #

lot #

date alqt

ul/aliquot

notes





AGE

Biorbyt

orb10064

A1268

5/7/14

25ul



CSPG (CS-56)

Sigma

C8035

032M4824

5/19/14

20ul

retest 2/2017

CSPG (CS-56)

Sigma

C8035

026M4834V

need aliq. (2x200ul); retest 10/2020

Alk Phos-ALPL

abcam

ab17973

GR157978-1

8/15/14

10ul



Osterix/Sp7

abcam

ab22552

GR138927-1

5/30/14

10ul

retest 5/28/19

Osterix/Sp7

abcam

ab22552

GR176974-2

3/5/15

10ul






















Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC

Premier Laboratory, LLC

PO Box 18592

Boulder, CO 80308

(303) 682-3949 office

(303) 682-9060 fax

(303) 881-0763 cell

liz at premierlab.com

www.premierlab.com



Ship to Address:



Premier Laboratory, LLC

1567 Skyway Drive, Unit E

Longmont, CO 80504





-----Original Message-----
From: Rene J Buesa via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Tuesday, August 16, 2016 9:31 AM
To: Julio Benavides; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Antibodies storage tips



I used to have several compartmented plastic alphabetized boxes with enough empty spaces to accommodate "new arrivals". If the spaces were used-up I just added a new box. Since they occupied several shelves, each shelf had the lettering identifying the boxes in each one.René



    On Tuesday, August 16, 2016 6:29 AM, Julio Benavides via Histonet <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:



Hi all,



In our lab we carry out quite a number of IHCs for research purposes.

The number of mAbs and pAb stored in the frezeer is getting to the point that, when you need to do and IHC, you actually lose more time diving through the frezeer draws for the right vial than doing the IHC! is not a question than we are running our of freezer room, it is more to the point of having a proper and standarized way to keep the antibodies and than produce a proper registry of what we have and where to find it.



I was wondering, in an attempt to avoid reinventing the wheel, if you could be as kind as to share your tips for storing and organizing the antibodies (aliquots, stock solutions, intermediate dilutions....) so chaos does not take over the lab!



As always, thank you very much for your time and advice!



Cheers



Julio





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