[Histonet] Histonet Digest, Vol 153, Issue 3

[Steve McClain]

Never had one of fancy new ones! But I have  one V5 and 4 K Sakuras. Those back to the future Delorean stainless steel  models run circles around the already rusting needs to be painted 8 year old model down more in its short life than all 4 flux capacitor Ks put together.  

Start by analyzing your history.
What tissue tissues are you processing?
How long is your cycle?
What clearant-xylene?
How long is your cleaning cycle?
What tissue wrapping are you using?

Steve A. McClain, MD

> On Aug 3, 2016, at 13:15, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
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> Today's Topics:
> 
>   1. Wax clogs in VIP 6 processor (cynthia haynes)
>   2. Re: Histonet Digest, Vol 153, Issue 2 microtomes (Steve McClain)
>   3. slides on the Benchmark Ultra (Amy Johnson)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Tue, 2 Aug 2016 18:49:17 +0000 (UTC)
> From: cynthia haynes <naje1972 at yahoo.com>
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Wax clogs in VIP 6 processor
> Message-ID:
>    <1833276652.9872747.1470163757288.JavaMail.yahoo at mail.yahoo.com>
> Content-Type: text/plain; charset=UTF-8
> 
> Hello Histo NetI am having a problem with wax clogs on my VIP 6 processor. This unit vents to the outside and I was just wondering if that may?have something to do with my problem. It started 2 weeks ago. I've had the unit serviced?2 x's. It has been very humid in our city. I am?changing sol'n regularly as per company supplied maintenance program. HELP!. I am at my wits end. This relatively a new unit. 3 year old. Has anyone experience this problem before.Thanks in advance.Cynthia Haynes-James H.T.
> 
> ------------------------------
> 
> Message: 2
> Date: Tue, 2 Aug 2016 18:56:51 +0000
> From: Steve McClain <SteveM at mcclainlab.com>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Histonet Digest, Vol 153, Issue 2 microtomes
> Message-ID: <A2D73415-8C93-42BF-A3BD-AF4DD24D94C8 at mcclainlab.com>
> Content-Type: text/plain; charset="us-ascii"
> 
> Depends on how long you plan to be in business and whether you are using other people's money.  But buy all the same model and make if you can.
> 
> I've owned the same Leica 2125 and 2135 microtomes for 12 years.  
> We usually cut mainly small biopsies but have pushed those to occasionally cut large  research blocks up to 45mm by 60mm.
> I cannot speak to other models. Since I've owned Leica, I haven't had any other experience. 
> 
> Like all mechanical equipment, maintenance is critical or none will cut properly.
> Steve A. McClain, MD
> 
>> On Aug 2, 2016, at 13:19, "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu> wrote:
>> 
>> Thanks to everyone who helped me out by providing their opinions on
>> embedding centers.  This time, I need everyone's thoughts on microtomes.
>> My lab is debating between the Thermo/Microm HM355S and the Leica RM2255.
>> Your thoughts and advice are very much appreciated!  If there are any more
>> I should try, let me know!
>> 
>> Thanks again,
>> Mary Faith
> 
> 
> 
> ------------------------------
> 
> Message: 3
> Date: Wed, 3 Aug 2016 14:06:09 +0000
> From: Amy Johnson <AJohnson at aipathology.com>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] slides on the Benchmark Ultra
> Message-ID:
>    <D2A2F4D6FC62CB47A21B179D1812DC52376B75DD at SERV2012.aipathology.local>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hello Histonetters,
> 
> WE currently run the Benchmark Ultra for our IHC staining.  We have been using SuperFrost Plus slides on the recommendation by Ventana.  What are others using if you aren't using SuperFrost Plus slides.
> Our vendor that we get SuperFrost Plus from no longer carries them and has replaced them with another type......Just wondering what others think
> 
> 
> Thanks
> Amylin Johnson, B.S. HTL(ASCP)
> Associates in Pathology
> Wausau Wi 54401
> 715-847-2130
> 
> 
> 
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> ------------------------------
> 
> End of Histonet Digest, Vol 153, Issue 3
> ****************************************