[Histonet] Reprocessing

[Gayle Callis]

A quick technique, published in Histologic Vol XXXVI, No. 1, May 2003    http://www.sakura-americas.com/Histologic/Histo-Tips/1008.htmlby   

Michael Johnson,  A technic for correcting poorly processed paraffin blocks.    

Melt the paraffin, blot to remove excess melted paraffin, place in cassette.  Put on processor with rest of tissues sitting in NBF for daily processing run. 

This way you don't have to go through the agony of trying going back and then forward through reagents and the tissues will be less damaged by more heating and exposure to drying reagents.  

Good luck

Gayle M. Callis
HTL/HT/MT(ASCP) 



-----Original Message-----
From: Mca Werdler via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, April 07, 2016 9:02 AM
To: Charles Riley <criley at dpspa.com>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Reprocessing

What i would do, melt the blocks you made.

1. Put them back in paraffin for one hour 2. Put them back in another paraffin or one hour 3. Put them in xylene for one hour 4. Put them in another xylene for one hour 5. Put them in another xylene for one hour 6. Put them back in paraffin for one hour 7. Put them back  in paraffin for one hour.

It takes some time. The reason why it was dificult to cut is because the alcohol and paraffin dont mix, there is a possibility that the parafin was not well enough impregnated in the tissue.
With the above solution, you can restore that.

Maarten

UNAM neurobiologia, histología
Mexico

2016-04-07 4:43 GMT-05:00 Charles Riley via Histonet <
histonet at lists.utsouthwestern.edu>:

> Hello all,
>
>  We had an issue with our processor the other night. Someone 
> accidentally put 100% alochol into the last xylene station. The tissue 
> sections were difficult to cut and or were unreadable. What is the 
> best way to reprocess them?  They are mostly small GI biopsies and 
> only two larger specimens
>
> --
>
> Charles Riley HT(ASCP)CM
>
> Histopathology Coordinator/ Mohs
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