[Histonet] Validating IHC on decalcified specimens

[Elizabeth Chlipala]

Teri

We have a spot on all of our IHC SOP's that will state if the antibody has been tested on decalcified samples.   We do not routinely add decalcified tissue to our routine IHC protocol development runs, so for example if the target is a soft tissue target we will develop on a variety of soft tissues that express different  staining intensities.  If the target is expressed in bone and decalcified bone is the target tissue we will work with both decalcified and soft tissue during the development process.    If we are taking a previously soft tissue antibody and testing it in bone or a sample that has been decalcified then that information is added to our internal tracking documents and then the SOP as a revision, we follow the same protocol if we are testing on another species that we have not previously stained for.  

I can give you an example of the last scenario.  We are frequently requested to run GFAP IHC sometimes the samples are routinely fixed on other occasions the skull has been left intact and the sample decaled, we initially developed and verified on soft tissue but later added to the verification that the antibody was tested on decaled samples and we would list the decalcification method/s tested in the SOP.  I'll send off examples of both our GLP and non-GLP IHC protocols in a different e-mail.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

Ship to Address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Tuesday, April 05, 2016 12:50 PM
To: 'histonet at lists.utsouthwestern.edu'
Subject: [Histonet] Validating IHC on decalcified specimens

Hi Histonetters,

Can someone tell me if they do anything specific to validate their IHC markers on decalcified specimens? If no, do you test them anyway?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohnson at genoptix.com<mailto:tejohnson at genoptix.com>
www.genoptix.com<http://www.genoptix.com/>


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