[Histonet] Fungus contamination on AFB slides

[Tony Henwood (SCHN)]

Hi Allison,
You probably need to track down the source of the Candida.
I would suggest you cytocentrifuge each solution, air-dry the cytospin slides and stain with a giemsa.
Most fungi (esp candida) will show up.
See:

Henwood A (2013) "Fungal Contamination of Hanks Solution" Diagnostic Cytopathology 41(5):453-455

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: Scott, Allison D via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Thursday, 24 September 2015 8:30 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Fungus contamination on AFB slides

Hello to all in histoland.   We are currently having a problem with candida on our AFB slides.  We are doing the stain by hand due to the unavailability of the AFB kits from ventana.  We are using bottled sterile water for rinses and the slides are not being put in coplin jars for staining.  All solutions are being pipetted on to the slides.  All alcohols are fresh and the kit which is from American MasterTech is new, just opened.  This by the way is a cytology case(cystospin). Containers to run down to water have been bleached.  Any suggetions where to go next.


Allison Scott
LBJ Hospital
Houston, TExas
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