[Histonet] Fungus contamination on AFB slides

[Scott, Allison D]

Hello to all in histoland.   We are currently having a problem with candida on our AFB slides.  We are doing the stain by hand due to the unavailability of the AFB kits from ventana.  We are using bottled sterile water for rinses and the slides are not being put in coplin jars for staining.  All solutions are being pipetted on to the slides.  All alcohols are fresh and the kit which is from American MasterTech is new, just opened.  This by the way is a cytology case(cystospin). Containers to run down to water have been bleached.  Any suggetions where to go next.


Allison Scott
LBJ Hospital
Houston, TExas
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