[Histonet] More on H202 issues

[Amos Brooks]

Hi,
     Peroxidase can really be a pain. If you look in the archives though
(or ask her really nice) Gayle Callis submitted a recipe for a glucose
oxidase for peroxidase quenching that does not include hydrogen peroxide.
If you aren't really a fan of making these things up I would bet dimes to
doughnuts that the recipe is *really* similar to the product from Biocare
Medical called PeroxAbolish.
Here's a link... http://biocare.net/product/peroxabolish/
I have used it and it did work, but I don't really use it regularly so
can't really compare it well. Incidentally if I am wrong in my assumptions
about the similarity of this to the glucose oxidase, I trust someone here
(even from the company itself) will gently correct me.

Cheers,
Amos

On Sat, Sep 12, 2015 at 1:00 PM, <histonet-request at lists.utsouthwestern.edu>
wrote:

> Message: 5
> Date: Fri, 11 Sep 2015 20:33:45 +0000
> From: "Lewis, Patrick" <patrick.lewis at seattlechildrens.org>
> To: " (Histonet at lists.utsouthwestern.edu)"
>         <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] More on H202 issues
> Message-ID:
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> <3903BE18914F4440834F0E620415FFCA3CBA0D27 at PPWEXD01d.childrens.sea.kids>
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>
> Hi Everyone
>
> Thanks for your responses.
>
> I am looking at cell surface markers,
> Sorry I should have said.
>
> Based on what I found out below:
>
> Methanol is out, even though I agree that Methanol does enhance the effect
> of H202 blocking.
> (I suppose I could try it to see how much/if any epitope loss there is in
> relation to H202 quenching,  At least It would help identifying false
> positives that are actually H202 artifacts.)
>
> Also it looks like increasing the concentration of H202 is out.
>
> As to when though,
>
> It looks like with cell surface markers I should block after the primary,
> or even after the 2ndary?
> Can I do that successfully with a HRP labeled 2ndary?
>
> thoughts?
>
> Patrick.
>