[Histonet] Xylene and Formalin substitutes

WILLIAM DESALVO wdesalvo.cac at outlook.com
Wed Oct 21 18:10:49 CDT 2015

Contact Ada Feldman, Anatech Ltd. Best resource.

Sent from my Windows Phone
From: Bharti Parihar via Histonet<mailto:histonet at lists.utsouthwestern.edu>
Sent: ‎10/‎21/‎2015 3:49 PM
To: histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Xylene and Formalin substitutes

Hello everyone. I'm interested in starting a conversation at my workplace
regarding substitutes for xylene,  and if there are any substitutes for
formalin. If anyone out there is using any can they please give me feedback
regarding the following questions,

1. Name of substitutes?
2. Pros and Cons?
3. Any quality issues when performing H&E, IHC or Special Stains?
4. Is it cost effective?
5. Does it require different dehydrants other than alcohol?
6. If formalin is still used as fixative,  what xylene substitute works
best with formalin?

I appreciate any feedback! Thanks!
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