[Histonet] Microtome suggestions
Hawkins, Hal K.
hhawkins at UTMB.EDU
Wed Oct 7 13:05:58 CDT 2015
The old Sorvall JB-4 microtome was designed for this purpose and works well. They are available at a reasonable price from several used equipment providers and on Ebay.
From: Goetz, Freya E. via Histonet [histonet at lists.utsouthwestern.edu]
Sent: Wednesday, October 07, 2015 11:07 AM
To: Rene J Buesa
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Microtome suggestions
Hi Rene and histonet,
Thank you for your suggestions. I must amend my earlier request. We have an ultra microtome from RMC and a Spencer 820 microtome. I would like to section epoxy embedded material that is larger than an ultramicrotome could handle but smaller than a sledge microtome. Sorry I wasn’t specific.
Thank you again!
On Oct 7, 2015, at 12:01 PM, Rene J Buesa <rjbuesa at yahoo.com<mailto:rjbuesa at yahoo.com>> wrote:
For large specimens you need a "horizontal" or sledge microtome. Leitz (Leica) manufactures the best, but you could try an OMS from Reichert although I am not sure they stll are manufactured because Leica swallowed Reichert some years ago.
Another alternative, and probably even better than the Leitz (Leica), is a Spencer (American Optical) sliding microtome Model 860
For me this is the best all-around ever manufactured sliding microtome and not too long ago a saw one for sale in e-Bay.
Why don't you try looking for it. It will be much cheaper than any modern horizontal microtome and far better than any new one.
On Wednesday, October 7, 2015 9:08 AM, "Goetz, Freya E. via Histonet" <histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>> wrote:
I was wondering if anyone has microtome opinions they would like to share. We have an ultramicrotome but I am looking for a microtome that could cut larger pieces of tissue embedded in plastic. I am hesitant to go with Leica because service is so expensive but I’m looking for honest opinions.
Thanks and have a nice day!
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