[Histonet] Tempature Logging
Brent Adams
badams at acadianagastro.com
Fri Oct 2 12:41:39 CDT 2015
Karen,
We were hit with minor incident by CLIA for not having Temp and Humidity documentation as well.
We also use the equipment manual to set the Temp and Humidity operation ranges.
WE have a hi/ lo Temp and Humidity monitor on the door jam between both rooms.
We hand document the time and the Tech signs off we * any day we have out of range
and write down the corrective action which is usually notified maintenance.
We also only document during hours of operation. Histology does not run 24/7/365 like the clinical Lab
so we have to adapt some of the rules to our operation.
Our block file room has a back up A/C unit so
Brent Adams – BS, LPN, HT
www.acadianagastro.com
Acadiana Gastroenterology Associates, LLC
439 Heymann Blvd
Lafayette, LA 70503
tel: (337) 269-1126
fax: (337) 269-1476
________________________________________
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu>
Sent: Friday, October 2, 2015 12:00 PM
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Subject: Histonet Digest, Vol 143, Issue 2
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Today's Topics:
1. 2016 FSH Annual Meeting Abstract Request (John Shelley)
2. Temperature logging (Heckford, Karen - SMMC-SF)
3. billing consults (Horn, Hazel V)
4. Re: Temperature logging (Elizabeth Chlipala)
5. Opinions about the Intavis InsituPro system (Sylvain Loub?ry)
6. autoradiography frozen human brain sections fall off, white
matter issue (Kooijman, Esther)
7. GMS and Gram stains (Histology)
8. Re: autoradiography frozen human brain sections fall off,
white matter issue (Connolly, Brett M)
9. Labeling formats (Stephen G. Ruby)
10. Missing specimen (Jennifer Clark)
11. Re: Labeling formats (Hannen, Valerie)
----------------------------------------------------------------------
Message: 1
Date: Thu, 1 Oct 2015 17:27:08 +0000
From: John Shelley <jshelley at sbpdiscovery.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] 2016 FSH Annual Meeting Abstract Request
Message-ID:
<C54F513DA7DA7547B37103A4B74BDAE903DE44FD at CARRERA.ln.burnham.org>
Content-Type: text/plain; charset="us-ascii"
Good Morning,
I know that there are many people out there that have a passion to teach what they know and we would very much like to put a call out to those who would be interested in presenting a workshop or two at our 2016 Florida Society for Histotechnology Annual meeting in the beautiful gulf coast area of St. Petersburg FL on May19-22, 2016 at the St. Pete Bayfront Hilton. We will have two and half days' worth of lecture time, starting with our popular Administrator and Supervisor Forum, the whole day HT preparedness workshop for students or those who are planning on taking their HT/HTL certification through ASCP. Please consider this great venue and submit your abstracts.
We are interested in topics on special stains, IHC, In Situ, Lean, billing, safety, digital pathology, ergonomics, cytology, CAP/CLIA inspection tips, leadership and any other topic that is relevant to today's histology laboratory. Please send abstract to John Shelley at fshgrouppresident at gmail.com<mailto:fshgrouppresident at gmail.com>. I look forward to your submissions. Vendor submissions welcomed!
Our plan will be to notify you by late December to early January so please keep your calendars open until you hear back from us.
Kind Regards!
John J Shelley
2014 - 2016 FSH President
------------------------------
Message: 2
Date: Thu, 1 Oct 2015 10:42:57 -0700
From: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Temperature logging
Message-ID:
<D329219D3B967447A9E3ED3242117E320728DE5166 at PHX-MSG-007-N1.chw.edu>
Content-Type: text/plain; charset="us-ascii"
Good Morning,
I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this.
1st Question: What is your temperature range?
2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart?
The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it.
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford at dignityhealth.org
Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
------------------------------
Message: 3
Date: Thu, 1 Oct 2015 12:52:24 -0500
From: "Horn, Hazel V" <HornHV at archildrens.org>
To: "histonet (histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] billing consults
Message-ID:
<25A4DE08332B19499904459F00AAACB71A3BC790E9 at EVS1.archildrens.org>
Content-Type: text/plain; charset="us-ascii"
Histonetters,
1. Please see the questions about billing for Pathology Consultations under varied circumstances as denoted below. For each circumstance, please specify whether you bill,
a) professional and technical only, or
b) professional, technical and consult.
1. If your response choice is b), please specify whether your payors typically pay for the consult charge.
==============================================================================================================
1. The pathologist to whom the case was assigned has some doubts as to the best diagnosis after all attempts to classify the lesion.
2. The department has some disagreement about the diagnosis and sends the case to another pathologist to help decide the matter.
3. The clinician and/or patient requests a second opinion from another pathologist.
Hazel Horn
Supervisor of Histology/Autopsy/Transcription
Anatomic Pathology
Arkansas Children's Hospital
1 Children's Way | Slot 820| Little Rock, AR 72202
501.364.4240 direct | 501.364.1241 fax
hornhv at archildrens.org<mailto:hornhv at archildrens.org>
archildrens.org<http://www.archildrens.org/>
------------------------------
Message: 4
Date: Thu, 1 Oct 2015 12:16:04 -0600
From: Elizabeth Chlipala <liz at premierlab.com>
To: "Heckford, Karen - SMMC-SF" <Karen.Heckford at DignityHealth.org>
Cc: "'histonet at lists.utsouthwestern.edu'
(histonet at lists.utsouthwestern.edu)"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Temperature logging
Message-ID:
<14E2C6176416974295479C64A11CB9AE02230F9D95A4 at SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"
Karen
There was a similar question on temp charts the other day on the histonet. We record our temps daily on a form. Our temperature range is determined by the equipment in the room, all operating manuals have ranges listed for temp, we list all major equipment in a room and what the recommended temperature range is listed in the operating manual and make a determination of a range based upon how critical the equipment is, etc. We have an SOP that governs this and how we manage the thermometers also.
Liz
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
-----Original Message-----
From: Heckford, Karen - SMMC-SF via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, October 01, 2015 11:43 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Temperature logging
Good Morning,
I have been asked to monitor the room temperatures in the Histology lab, and anywhere we store paraffin blocks, slides and reagents. Some of you I know already do this.
1st Question: What is your temperature range?
2nd Question: Are you physically monitoring it daily and writing it down on a temperature chart?
The reason why Question 2 was I purchased these new thermometers that I can put in the Hi and Lo. It also has where I can download it on to a scan disk so I can down load and put on a flash drive or store in the computer. I am not sure why these will not work but I am getting grief from my Lab Director that is a Med Tech. I figure if it alarms on me I can down load it and figure out the time of the out of range since I am not here on the weekend. Cannot have anyone else do it because I am the only Histology person in this hospital. The lab people do not give much support when a alarm is going off they ignore it.
Karen Heckford HT ASCP CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
karen.heckford at dignityhealth.org
Caution: This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error. Any further review, dissemination, distribution, or copying of this message is strictly prohibited. If you have received this communication in error, please notify us immediately by reply email. Thank you."
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
------------------------------
Message: 5
Date: Fri, 2 Oct 2015 09:55:56 +0200
From: Sylvain Loub?ry <sylvain.loubery at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Opinions about the Intavis InsituPro system
Message-ID:
<CAH7qQEO2Wqm2Dt0oT65sWh-hT4keTcCO24ivzf+pG5AAAkhkxg at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Dear All,
my department is considering the purchase of an Intavis InsituPro VSi
system (http://intavis.com/products/automated-ish-and-ihc/insituprovsi),
and I'd be very interested in any feedback I could get on this instrument.
Our interests are in plant biology, for both immunofluorescence and in situ
hybridization stainings, and on whole-mount samples as well as microtome or
vibratome sections. Possibly to perform screens, but probably mostly for
routine work.
If anyone has any opinions about this system, I'd be very grateful for any
piece of information I could get! (warnings, possibilities and limitations,
particular things to pay attention to...)
Thanks!
Sylvain
Sylvain Loub?ry, Ph.D.
Plant Imaging Unit
Department of Botany and Plant Biology
University of Geneva / Sciences III
Quai Ernest-Ansermet 30
1211 Geneva 4
Switzerland
Phone: (022.37.)96568
sylvain.loubery at unige.ch
http://biveg.unige.ch/services/imagerie.html
------------------------------
Message: 6
Date: Fri, 2 Oct 2015 09:19:47 +0000
From: "Kooijman, Esther" <e.kooijman at vumc.nl>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] autoradiography frozen human brain sections fall
off, white matter issue
Message-ID:
<51AEA3C85E64ED4E851909E3CCAB574CC9A84F57 at SP-MX-MBX2.vumc.nl>
Content-Type: text/plain; charset="us-ascii"
Dear Histonetters,
Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ?
We have problems with falling off sections of human brain tissue.
We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography :
Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica.
Stored in -20.
Day of the experiment.
Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes.
About 30 minutes - an hour drying at room temperature.
Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue.
Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute.
What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me.
Would Superfrost plus gold glass be better ?
Kind Regards,
Esther Kooijman
Research Technician
VU University Medical Center
Nuclear Medicine & PET Research
Email: e.kooijman at vumc.nl<mailto:a.metaxas at vumc.nl>
Amsterdam
The Netherlands
------------------------------
Message: 7
Date: Fri, 2 Oct 2015 11:59:10 +0000
From: Histology <histo at pathlab.us>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] GMS and Gram stains
Message-ID: <566E7795BC94204D9A21539DE282AED73098F0E5 at DC01.dp.local>
Content-Type: text/plain; charset="us-ascii"
Hi All Netters,
I need to get a better GMS and Gram stain. If anyone could share their protocols where they get the solutions that would be fantastic.
Thanks,
Mehndi Helgren
Dominion Pathology Labs
Norfolk, VA (AKA Hurricane Central) :)
------------------------------
Message: 8
Date: Fri, 2 Oct 2015 10:07:45 -0400
From: "Connolly, Brett M" <brett_connolly at merck.com>
To: "Kooijman, Esther" <e.kooijman at vumc.nl>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] autoradiography frozen human brain sections
fall off, white matter issue
Message-ID:
<C01C35B84DCDCE49BC60867E87F1C8FE010CB020DE99 at USCTMXP51015.merck.com>
Content-Type: text/plain; charset="us-ascii"
Hi Esther,
We do a lot of autoradiography on rodent, primate and human brains sections. Our protocol is similar to yours except we do not dry the slides after incubating in the buffer and before adding the radiotracer.
The experiment is performed with the slides in a rack and totally immersed in the solutions in staining dishes.
1. incubate slides in assay buffer 15 min. at RT
2. add radiotracer to buffer, gently mix and incubate 90-120 min. at RT
3. wash GENTLY (no agitation) in ice-cold wash buffer 3 x 3 min. We set up 3 containers and transfer the slides.
4. wash in ice-cold DI water 5 seconds.
5. air dry
The Superfrost gold slides are supposed to improve adherence and would be worth a try. It could be your other Superfrost slides are old or a bad batch...which I think has been experienced by some people on the list.
We do store our sectioned slides at -70C until use and then bring them up to RT the day of the experiment. They are sectioned, dried at RT for 15-20 min, and then store at -70C... No drying in the fridge.
Good luck,
Brett
Brett M. Connolly, Ph.D.
Principle Scientist,
Translational Biomarkers - Imaging
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly at merck.com
T- 215-652-2501
F- 215-993-6803
-----Original Message-----
From: Kooijman, Esther via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, October 02, 2015 5:20 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] autoradiography frozen human brain sections fall off, white matter issue
Importance: High
Dear Histonetters,
Does anyone of you have experience in autoradiography and could help me with the problem falling of tissue ? Or suggestions ?
We have problems with falling off sections of human brain tissue.
We have done the same experiments and cutting in rodent tissue without any problems. Our protocol for autoradiography :
Snap frozen tissue, cut in cryo 20um sections on Superfrost plus glass, drying ON in the fridge on silica.
Stored in -20.
Day of the experiment.
Getting them on room temperature about 45 minutes, washing/dehydrate on room temperature in 5mM Tris (HCL PH7.4) buffer, 20 minutes.
About 30 minutes - an hour drying at room temperature.
Then incubation with the radio tracer for 30 minutes, room temperature, just dripping the 1 mL solution to completely cover the superfrost plus glass and ditto tissue.
Turning the glass to get the solution of the glass, dipping in ice cold tris washing buffer 5mM (HCL PH7.4) 1.5 minutes and here the disaster starts with falling off extensive white matter falling off...... its washing steps of only 1.5 minute.
What can I do to prevent this, any idea ? I am out of clues. I have tried to dry the sections longer after cutting sections, I have tried to pre wash in either room temp or cold buffer. Hope someone can help me.
Would Superfrost plus gold glass be better ?
Kind Regards,
Esther Kooijman
Research Technician
VU University Medical Center
Nuclear Medicine & PET Research
Email: e.kooijman at vumc.nl<mailto:a.metaxas at vumc.nl>
Amsterdam
The Netherlands
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Message: 9
Date: Fri, 2 Oct 2015 09:09:50 -0500
From: "Stephen G. Ruby" <Sruby at 4path.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Labeling formats
Message-ID: <92B59D6A-774A-428C-AD5F-A3B458CEF7D8 at 4path.com>
Content-Type: text/plain; charset="us-ascii"
I am curious on what formats that labs use for labeling.
We use prefix-year-accession number-part(alpha)-block number(numeric)
Example. S15-12345-A1
If you use a different format can you share it here?
Thanks.
Dr Ruby
Sent from my mobile device
------------------------------
Message: 10
Date: Fri, 2 Oct 2015 10:47:38 -0400
From: Jennifer Clark <jennc976 at gmail.com>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Missing specimen
Message-ID: <2C4E8BAA-B34E-4D7E-868B-FB51CC9E14D0 at gmail.com>
Content-Type: text/plain; charset=us-ascii
I have a situation that has occurred and am unsure how to proceed. One MA called to get results for a young patient. We had no record of a specimen for this patient at all. The doctor was sure it was performed. With some research, we discovered that another patient, middle aged,(who was in the same room with the same MA after the young patient) had two tissue pieces in his specimen bottle. This was not mentioned in the original path report, and the tissue was sent for a second opinion to confirm a malignancy. Upon re-reviewing the slide, the two specimens in the middle aged patient's block look different, one malignant, one benign. Also one showed "young skin" and the other sun damaged, more mature skin, according to the dermatologist.
They are now wanting these tissues split up and a path report issued on the young patient who's specimen is missing based on these findings, with his being the benign tissue. I feel, while suspicious, I should not do this with out DNA testing to confirm. That being said, to my knowledge no other specimens are missing from that day. Please Help!
Thanks!
Jennifer Clark, HT
Sent from my iPhone
------------------------------
Message: 11
Date: Fri, 2 Oct 2015 10:56:11 -0400
From: "Hannen, Valerie" <Valerie.Hannen at parrishmed.com>
To: "'Stephen G. Ruby'" <Sruby at 4path.com>
Cc: "Histonet at lists.utsouthwestern.edu"
<Histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Labeling formats
Message-ID: <450B7A81EDA0C54E97C53D60F00776C32347EC18E1 at isexstore03>
Content-Type: text/plain; charset="us-ascii"
Ours is very similar...only difference being ours is: 15S-1234A-1 (year-prefix-accession number-part(alpha)-block number(numeric).
Valerie Hannen,MLT(ASCP),HTL,SU (FL)
Section Chief, Histology
Parrish Medical Center
951 N. Washington Ave.
Titusville,Florida 32796
T: (321)268-6333 ext. 7506
F: (321) 268-6149
valerie.hannen at parrishmed.com
www.parrishmed.com
-----Original Message-----
From: Stephen G. Ruby via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Friday, October 02, 2015 10:10 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Labeling formats
I am curious on what formats that labs use for labeling.
We use prefix-year-accession number-part(alpha)-block number(numeric) Example. S15-12345-A1
If you use a different format can you share it here?
Thanks.
Dr Ruby
Sent from my mobile device
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