[Histonet] DIF on paraffin embedded tissue

[Elizabeth Chlipala]

Teri

Excellent point and to add to that most IF techniques that are employed on FFPE are not direct they are indirect and consist of an unlabeled primary and then a secondary that is conjugated with an alexa fluor or something similar at least that is how we approach IF on FFPE tissues here.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
liz at premierlab.com
www.premierlab.com

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-----Original Message-----
From: Teri Johnson via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Sent: Wednesday, November 25, 2015 11:30 AM
To: 'histonet at lists.utsouthwestern.edu'
Subject: Re: [Histonet] DIF on paraffin embedded tissue

Maryann,

Whoa, bad day. I'm sorry for you and for the patient.

As for whether the testing usually performed in frozen section by Direct ImmunoFluorescence can be successfully done on FFPE, Liz Chlipala is probably the closest to answering this. This was likely a kidney or skin biopsy. The panel is likely something like IgG, IgA, IgM, C3, Fibrinogen and maybe another marker or so.

Can it be done? I would say not with any degree of confidence unless this was extensively tested side-by-side with frozen vs paraffin embedded tissues. The IHC stain techniques would be radically different. This would take a lot of time.

Now, if you were successful in providing these assays that you have validated in your laboratory (I shudder to think what that would entail), would the pathologist be comfortable signing out a case such as this? Would the clinician be comfortable treating the patient based on these results?

Teri Johnson
Manager, Clinical Trial Testing
Genoptix, Inc., a Novartis company
BioPharma
1811 Aston Avenue
Carlsbad, CA  92008
USA

Phone +1 760 516 5954
tejohnson at genoptix.com<mailto:tejohnson at genoptix.com>
www.genoptix.com<http://www.genoptix.com/>


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