[Histonet] Mohs sections

[Angela Yepes]

> From: Angela Yepes <angelapatriciay at gmail.com>
> Date: November 12, 2015 at 6:05:57 PM EST
> To: histonet at lists.utsouthwestern.edu
> Subject: Mohs sections
> 
> 
>> From: Angela Yepes <angelapatriciay at gmail.com>
>> Date: November 12, 2015 at 4:15:50 PM EST
>> To: histonet-request at lists.utsouthwestern.edu
>> Subject: Mohs sections
>> Hello everyone:
>> While cutting Sections for Mohs surgery I noticed the tissue detaching from the O.C.T. Embedding medium  and subsequent rolling of the tissue.
>> To correct the problem the chuck was rotated to allow cutting from the opposite side (epidermis side). This maneuver did not correct the problem. I tried to unroll the edges with stiff brush which seemed to correct the problem, but it did not. I only  found out the sections were still rolling up after the staining.
>> The cryostat temperature was -23C and the sections thickness was -14 microns 
>> Please see pictures.
>> Please help 
>> 
>> 
>> 
>> 
>> Sent from my iPhone
>>