[Histonet] IHC on old slides

[Rene J Buesa]

The only thing you can do is to prolong the Ab staining time but, in order to avoid excessive background, dilute it. You will have to find an adequate balance between a more diluted Ab with a weaker spitope signal and a  prolonged incubation time and there is no "magic formula" for obtaining it. You will have to make tests.Antigen in stored slides oxidizes producing a weaker signal.René  


     On Friday, November 13, 2015 10:51 AM, "Stoll, Kathryn via Histonet" <histonet at lists.utsouthwestern.edu> wrote:
   

 Hi Everyone,

I have been doing some IHC staining on slides that are 20 years old or more.  Depending on the antibody the staining is pale in comparison to my control slide.  Does anyone have experience with staining older slides?  Do you have any tips to share?  The slide storage conditions are not something I can control.  I am under the assumption that they were stored at room temperature.
Thank you in advance.

Kathryn Stoll

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet