[Histonet] IHC on old slides

[Stoll, Kathryn]

Hi Everyone,

I have been doing some IHC staining on slides that are 20 years old or more.   Depending on the antibody the staining is pale in comparison to my control slide.  Does anyone have experience with staining older slides?  Do you have any tips to share?  The slide storage conditions are not something I can control.  I am under the assumption that they were stored at room temperature.
Thank you in advance.

Kathryn Stoll