[Histonet] Paraffin sectioning for DNA analysis

[P Sicurello]

Hello Listers,



Which techniques are you all using to avoid cross contamination when
cutting multiple sections from multiple blocks for DNA analysis?



Way back I used to use DEP-C treated water, wore gloves, changed the blade
between blocks and wiped down the surface of the knife holder plate with
the DEP-C water and ETOH.



What is the currently accepted procedure?



Sincerely,

Paula


Paula Sicurello, HTL (ASCP)CM

Histotechnology Specialist

UC San Diego Health

200 Arbor Drive

San Diego, CA 92103

(P): 619-543-2872



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