[Histonet] TRAP Stain Help
Mack, Sarah
Sarah_Mack at urmc.rochester.edu
Tue May 12 12:04:20 CDT 2015
Travis,
Were your samples stored in alcohol? If so, that can kill the TRAP enzyme. Your protocol is similar to ours.
Sarah Mack
University of Rochester Medical Center
Center for Musculoskeletal Research
Histology, Biochemistry, and Molecular Imaging Core
601 Elmwood Avenue
Box 665
Rochester, NY 14642
(585)-273-3901
________________________________________
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Sent: Tuesday, May 12, 2015 1:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 138, Issue 14
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Today's Topics:
1. anti-human IgG antibody (Krempley, Amanda L)
2. TRAP Stain Help (Wait, Trevor Jordan)
3. Per Diem Positions at UC San Diego Health System (Paula Sicurello)
4. H&E Stainer Question (Paula Sicurello)
5. Re: H&E Stainer Question (Roy, Lisa)
6. Re: H&E Stainer Question (Sanders, Jeanine (CDC/OID/NCEZID))
7. Re: H&E Stainer Question (Simmons, Christopher)
8. Re: H&E Stainer Question (Roy, Lisa)
9. RELIA HOT JOB Alert! Lead Histotech needed for Brand New Lab
in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!! (Pam Barker)
10. Re: H&E Stainer Question (Joelle Weaver)
----------------------------------------------------------------------
Message: 1
Date: Mon, 11 May 2015 19:52:18 +0000
From: "Krempley, Amanda L" <amanda.krempley at abbvie.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] anti-human IgG antibody
Message-ID:
<9667996145fe4392bc5c71b3f3a8ae94 at USAASECSM048.R0018.COLLABORATION.ECS.HP.COM>
Content-Type: text/plain; charset="utf-8"
Hello Histoland,
Has anyone used an anti-human IgG antibody that does not cross react with Non-human primates, specifically cynomolgus, for IHC?
Could you please share the antibody and protocol that you have had success with?
Thank you,
Amanda
------------------------------
Message: 2
Date: Mon, 11 May 2015 20:26:14 +0000
From: "Wait, Trevor Jordan" <WaitT at livemail.uthscsa.edu>
To: "Histonet at Lists. Edu" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] TRAP Stain Help
Message-ID: <1431375976414.44600 at livemail.uthscsa.edu>
Content-Type: text/plain; charset="iso-8859-1"
Hey guys, recently I've used the Sigma Aldrich TRAP Stain Kit in order to stain for Osteoclasts in Formalin Fixed Paraffin Embedded bone tissues that are EDTA decalcified. Unfortunately there was no TRAP stain to be found whatsoever on all slides that I stained. I was hoping that someone with some experience with TRAP stain could really help me out! Here are a couple of reasons that I have wondered as to why the TRAP stain might not be visible....
1. Staining too long with Hematoxylin counterstain? I have noticed in several trial runs that sometimes if the Hematoxylin counterstain is too long then this can effect the amount of TRAP stain that shows up with the osteoclasts. Perhaps I'm just over analyzing....
2. Perhaps no osteoclasts were present to even stain....does anyone know what the usual ratio of Osteoclasts to Osteocytes are? In the past stains that I have used....whenever there is an osteoclasts that shows up...it is usually pretty spotty and many times they are in groups together...is this how it normally is?
3. Perhaps the TRAP stain is being washed away through the rinsing with dI or dehydration with ethanol/clearing just before mounting.
A protocol that I used before the Sigma Aldrich Kit incorporates incubating the slides in 37 celsius water for 1 hour just prior to allowing the slides to sit in the TRAP stain solution (this is also 37 celsius in the same water bath) and letting it sit for 20 minutes. With this stain...there seems to be a consistent showing of osteoclasts but I'm just not sure if all of the osteoclasts are showing up correctly...that is why I moved to the Sigma Aldrich kit to make sure and the results didn't show ANY osteoclasts there! Anyways...I'm just curious if there are any reasons that the TRAP stain is not showing up...I would appreciate any input!
Trevor Jordan Wait
University of Texas Health Science Center, San Antonio
Class of 2017 MD Candidate
------------------------------
Message: 3
Date: Mon, 11 May 2015 13:57:21 -0700
From: Paula Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Per Diem Positions at UC San Diego Health System
Message-ID:
<CAPSjddbK50rXrn2a3rYAg5CXTLKmqitym700UCo4WnHjbbzJJA at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Good Afternoon Listers,
UCSD Medical Center Department of Anatomic Pathology has two per diem
positions open in Histology.
Histology experience is a must. If you are interested email your resumes
to:
Craig Fisher at *cfisher at ucsd.edu <cfisher at ucsd.edu>*
and
Mike Mathhew at *mimatthew at ucsd.edu <mimatthew at ucsd.edu>*
Thank you and have a nice day.
Paula :-)
------------------------------
Message: 4
Date: Mon, 11 May 2015 14:03:40 -0700
From: Paula Sicurello <patpxs at gmail.com>
To: HistoNet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] H&E Stainer Question
Message-ID:
<CAPSjddakhd+ao9n0oOYaYJR5uqnnWaRD8Q5Qtv80sMYxfv_=2A at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Me again...
UCSD is in the market for a new H&E stainer for our new hospital opening
next year.
We need a workhorse, not a prima dona, something with a coverslipper built
in would be nice.
What do you use?
Suggestions gratefully accepted-even from you two Keith and Matt ;)
Opinions about the good, the bad, and the ugly (as long as it works really
well) will be helpful.
Thanks oodles!
Paula :-)
------------------------------
Message: 5
Date: Tue, 12 May 2015 12:18:46 +0000
From: "Roy, Lisa" <Royl1 at LabCorp.com>
To: Paula Sicurello <patpxs at gmail.com>, HistoNet
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] H&E Stainer Question
Message-ID:
<dbe036bd84184f478f759f210e463051 at rtwems04.lca.labcorp.com>
Content-Type: text/plain; charset="us-ascii"
Paula
Here are my two cents....
I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on.
On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results.
Hope it helps....good luck.
Lisa
-----Original Message-----
From: Paula Sicurello [mailto:patpxs at gmail.com]
Sent: Monday, May 11, 2015 5:04 PM
To: HistoNet
Subject: [Histonet] H&E Stainer Question
Me again...
UCSD is in the market for a new H&E stainer for our new hospital opening next year.
We need a workhorse, not a prima dona, something with a coverslipper built in would be nice.
What do you use?
Suggestions gratefully accepted-even from you two Keith and Matt ;)
Opinions about the good, the bad, and the ugly (as long as it works really
well) will be helpful.
Thanks oodles!
Paula :-)
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e=
-This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722.
------------------------------
Message: 6
Date: Tue, 12 May 2015 12:26:38 +0000
From: "Sanders, Jeanine (CDC/OID/NCEZID)" <jqb7 at cdc.gov>
To: "Roy, Lisa" <Royl1 at LabCorp.com>, Paula Sicurello
<patpxs at gmail.com>, HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] H&E Stainer Question
Message-ID:
<3B2CD438E1628A41BD687E98B963B78137F3A0D3 at EMBX-CLFT4.cdc.gov>
Content-Type: text/plain; charset="us-ascii"
This is what we have Leica) and it has 4 ovens. https://urldefense.proofpoint.com/v2/url?u=http-3A__www.leica-2Dmicrosystems.com_news-2Dmedia_news_news-2Ddetails_article_leica-2Dst5020-2Dmultistainer-2Dworkstation_&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=1Jk81pXE6-81arrP_81cZthjnCrrpONTB8phTSHW8hE&e=
We also have a Prisma and we like them both. We have had the Leica longer and it just never breaks down.....knock on wood! We have to have glass coverslips and the Prisma glass coverslipper is a bit more finicky than the Leica.
-----Original Message-----
From: Roy, Lisa [mailto:Royl1 at LabCorp.com]
Sent: Tuesday, May 12, 2015 8:19 AM
To: Paula Sicurello; HistoNet
Subject: Re: [Histonet] H&E Stainer Question
Paula
Here are my two cents....
I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on.
On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off
with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results.
Hope it helps....good luck.
Lisa
-----Original Message-----
From: Paula Sicurello [mailto:patpxs at gmail.com]
Sent: Monday, May 11, 2015 5:04 PM
To: HistoNet
Subject: [Histonet] H&E Stainer Question
Me again...
UCSD is in the market for a new H&E stainer for our new hospital opening next year.
We need a workhorse, not a prima dona, something with a coverslipper built in would be nice.
What do you use?
Suggestions gratefully accepted-even from you two Keith and Matt ;)
Opinions about the good, the bad, and the ugly (as long as it works really
well) will be helpful.
Thanks oodles!
Paula :-)
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e=
-This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722.
_______________________________________________
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------------------------------
Message: 7
Date: Tue, 12 May 2015 12:32:43 +0000
From: "Simmons, Christopher" <simmca at UPMC.EDU>
To: "'Roy, Lisa'" <Royl1 at LabCorp.com>, Paula Sicurello
<patpxs at gmail.com>, HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] H&E Stainer Question
Message-ID:
<6295CCF61B0DBB4C9248F51F8940E6A10118F112 at MSXMBXNSPRD09.acct.upmchs.net>
Content-Type: text/plain; charset="us-ascii"
To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+
You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes)
Then it goes much faster.
As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it.
CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years.
Chris Simmons B.S., A.S., HTL(ASCP)
Supervisor, UPP Dermatopathology
412.864.3880 office
412.612.0881 cell
-----Original Message-----
From: Roy, Lisa [mailto:Royl1 at LabCorp.com]
Sent: Tuesday, May 12, 2015 8:19 AM
To: Paula Sicurello; HistoNet
Subject: Re: [Histonet] H&E Stainer Question
Paula
Here are my two cents....
I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on.
On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results.
Hope it helps....good luck.
Lisa
-----Original Message-----
From: Paula Sicurello [mailto:patpxs at gmail.com]
Sent: Monday, May 11, 2015 5:04 PM
To: HistoNet
Subject: [Histonet] H&E Stainer Question
Me again...
UCSD is in the market for a new H&E stainer for our new hospital opening next year.
We need a workhorse, not a prima dona, something with a coverslipper built in would be nice.
What do you use?
Suggestions gratefully accepted-even from you two Keith and Matt ;)
Opinions about the good, the bad, and the ugly (as long as it works really
well) will be helpful.
Thanks oodles!
Paula :-)
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e=
-This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722.
_______________________________________________
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------------------------------
Message: 8
Date: Tue, 12 May 2015 12:36:49 +0000
From: "Roy, Lisa" <Royl1 at LabCorp.com>
To: "Simmons, Christopher" <simmca at UPMC.EDU>, Paula Sicurello
<patpxs at gmail.com>, HistoNet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] H&E Stainer Question
Message-ID:
<e550bb27cf4b4da3bcd1ec5309404145 at rtwems04.lca.labcorp.com>
Content-Type: text/plain; charset="us-ascii"
Depends on which model you have...hence XL. Ours only has one oven so you're really only getting 3-4 racks stained at a time.
-----Original Message-----
From: Simmons, Christopher [mailto:simmca at UPMC.EDU]
Sent: Tuesday, May 12, 2015 8:33 AM
To: Roy, Lisa; Paula Sicurello; HistoNet
Subject: RE: [Histonet] H&E Stainer Question
To be fair, a "batch" of slides for Leica is actually 270- slides, It can run 9 racks at a time, but, the 9th rack from start to coverslip is 3 hours+ You can always skip the on-board oven and place your slides in a slide dryer (most labs have them) and then every 3 minutes you can load a rack (1st xylene step 3 minutes) Then it goes much faster.
As for tape..ugh..it is only guaranteed to last 7 years, after that they pull off the slide and take the tissue with it.
CAP is starting to frown on this as you need to keep the initial H&E slides for up to 10+ years.
Chris Simmons B.S., A.S., HTL(ASCP)
Supervisor, UPP Dermatopathology
412.864.3880 office
412.612.0881 cell
-----Original Message-----
From: Roy, Lisa [mailto:Royl1 at LabCorp.com]
Sent: Tuesday, May 12, 2015 8:19 AM
To: Paula Sicurello; HistoNet
Subject: Re: [Histonet] H&E Stainer Question
Paula
Here are my two cents....
I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on.
On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results.
Hope it helps....good luck.
Lisa
-----Original Message-----
From: Paula Sicurello [mailto:patpxs at gmail.com]
Sent: Monday, May 11, 2015 5:04 PM
To: HistoNet
Subject: [Histonet] H&E Stainer Question
Me again...
UCSD is in the market for a new H&E stainer for our new hospital opening next year.
We need a workhorse, not a prima dona, something with a coverslipper built in would be nice.
What do you use?
Suggestions gratefully accepted-even from you two Keith and Matt ;)
Opinions about the good, the bad, and the ugly (as long as it works really
well) will be helpful.
Thanks oodles!
Paula :-)
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=4sF48jRmVAe_CH-k9mXYXEGfSnM3bY53YSKuLUQRxhA&r=ODw50OyFtFWu8REOenc_8wsdRMG_cbkreuWUix7iMVo&m=DR5hhSofRSHkcr9uY8sQ0Qfr0tmGbuJDCIwuTAylDyI&s=ojqKLAQtVhO8ixOrdIb13M0riQkWk2GB5BRQSgbZ3_Q&e=
-This e-mail and any attachments may contain CONFIDENTIAL information, including PROTECTED HEALTH INFORMATION. If you are not the intended recipient, any use or disclosure of this information is STRICTLY PROHIBITED; you are requested to delete this e-mail and any attachments, notify the sender immediately, and notify the LabCorp Privacy Officer at privacyofficer at labcorp.com or call (877) 23-HIPAA / (877) 234-4722.
_______________________________________________
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Message: 9
Date: Tue, 12 May 2015 10:21:37 -0400
From: "Pam Barker" <relia1 at earthlink.net>
To: "Histonet" <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] RELIA HOT JOB Alert! Lead Histotech needed for
Brand New Lab in Dallas/Ft. Worth. A RELIA EXCLUSIVE!!!
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Hi Histonetters!!
How are you?
I have an exciting opportunity that just might interest you.
OR
If you happen to know someone qualified who might be interested
I welcome you to refer them. If I place them you will earn a referral fee.
The position we have been engaged to work on is an ASCP Certified histotech
with dermpath experience (Mohs is a plus and my client will train).
This is for a BRAND NEW LAB located in the Dallas/Fort Worth area. This
person will be the sole practitioner histotech in this brand new lab.
You will be responsible for managing and maintaining the lab and performing
histology.
My client offers a competitive salary, nice benefits and an outstanding
opportunity.
For more information please contact me
Pam Barker at relia1 at earthlink.net or toll free at 866-607-3542.
Thanks-Pam
Right Place, Right Time, Right Move with RELIA!
Thank You!
Pam M. Barker
Pam Barker
President/Senior Recruiting Specialist-Histology
RELIA Solutions
Specialists in Allied Healthcare Recruiting
5703 Red Bug Lake Road #330
Winter Springs, FL 32708-4969
Phone: (407)657-2027
Cell: (407)353-5070
FAX: (407)678-2788
E-mail: relia1 at earthlink.net
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Message: 10
Date: Tue, 12 May 2015 16:26:58 +0000
From: Joelle Weaver <joelleweaver at hotmail.com>
To: "Roy, Lisa" <royl1 at labcorp.com>, Paula Sicurello
<patpxs at gmail.com>, "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] H&E Stainer Question
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Personally I love the Prisma for volume and the tape. I know many have bad opinions, but I wish I had both the Prisma and the tape right now! I have never seen any problems with very old ( 15+ year) slides. using the tape. Not saying it can't happen-but have not personally seen it. The tape is easier to get off if you need to versus old glass CS, just use acetone, acetone/xylene, xylene. Comes off in a gel form and slides right off leaving the tissue intact.
Joelle Weaver MAOM, HTL (ASCP) QIHC
> From: Royl1 at LabCorp.com
> To: patpxs at gmail.com; histonet at lists.utsouthwestern.edu
> Date: Tue, 12 May 2015 12:18:46 +0000
> Subject: Re: [Histonet] H&E Stainer Question
>
> Paula
> Here are my two cents....
>
> I currently use a Leica Autostainer XL with attached glass coverslipper. It is consistent in its staining and easy to use. The downfall is if you are a large volume lab or just have large volume days, each staining rack holds 30 slides and only one rack can be stained in each batch. The stainer also only has one on board oven, so the throughput of this machine is fairly low. It is only staining 30 slides at a time, with one holding station for the next set. It will run multiple batches concurrently, but gets to a point where it is all backed up. We sometimes have 2 racks staining, one in the oven, one in the loading dock, and some sitting on top of the stainer until it can go on.
>
> On the flip side, the Sakura Prisma is a workhorse. It is very similar to the Leica in the sense that it is linear and very consistent in staining. It has two on board ovens and each basket can hold 20 slides. The difference is that the Sakura can stain 3 racks (60 slides) per batch, with two batches in the oven at the same time. That gives you 120 slide throughput for each batch. This stainer also has an attached coverslipper (Sakura Film), but it is film coverslips. I know, I know.....no one likes the film coverslips. One advantage to the film, is that the slides are dry almost immediately and can be filed away the same day. No waiting for 3-4 days for the glass ones to fully cure. I can say that the last lab I worked in had the film coverslips and after 10 years, the slides were still in pristine condition. There are many pathologists that do not like to read film covered slides, but once ours got used to it, they had no problems. Some say the film yellows or comes off with the tissue still attached. I can say that I never seen this in my past position. It is very dependent on Xylene only during coverslipping. You cannot use a xylene substitute in the coverslip portion and expect to get good results.
>
> Hope it helps....good luck.
> Lisa
>
> -----Original Message-----
> From: Paula Sicurello [mailto:patpxs at gmail.com]
> Sent: Monday, May 11, 2015 5:04 PM
> To: HistoNet
> Subject: [Histonet] H&E Stainer Question
>
> Me again...
>
> UCSD is in the market for a new H&E stainer for our new hospital opening next year.
>
> We need a workhorse, not a prima dona, something with a coverslipper built in would be nice.
>
> What do you use?
>
> Suggestions gratefully accepted-even from you two Keith and Matt ;)
>
> Opinions about the good, the bad, and the ugly (as long as it works really
> well) will be helpful.
>
> Thanks oodles!
>
> Paula :-)
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>
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