[Histonet] optic chiasmata

[Salomao Segal]

I intend to use a cryostat to cut 70 - 100 micron thick sections of human
optic chiasmata.

Tissue is cryoprotected with 30% sucrose solution.

My question relates to the freezing process per se.

Would it be enough to place the tissue in a -4 freezer to harden and then
transfer to the cryostat chamber at say -20 wait a while and cut?  Or is it
necessary to introduce an intermediary step for freezing?

Thanks

SS


*Solomon Segal, M.D.*




*Associate Professor of Anatomy in SurgeryCenter for Anatomical Science and
Education (CASE)Department of SurgerySchool of MedicineSaint Louis
University*

*1402 South Grand Blvd.*
*Schwitalla Hall - 3rd Floor - M310*


*Saint Louis, MO, 63104office: 314 977 8023laboratory: 314 977 8080*


*CASE: 314 977 8027FAX: 314 977 5127e-mail*: ssegal2 at slu.edu

http://medschool.slu.edu/anatomy/

http://slu.academia.edu/SolomonSegal

https://sites.google.com/a/slu.edu/segal-laboratory/

https://sites.google.com/a/slu.edu/dr-segal-s-clinical-anatomy-website/