Fwd: [Histonet] Phosphotungstic acid

[John Kiernan]

Dear Cecilia,
I hadn't seen the 2009 paper you mention. Please let me know the reference. I've just sent you another email with an attached LM photo, which might be of interest.
John Kiernan
= = =
On 25/03/15, "Cecilia A."  <cecystan76 <@t> gmail.com> wrote:
> > 
> > Hi John,
> > 
> > Thank you for your reply.
> > 
> > In a Developmental Dynamics paper in 2009, PTA was used as a "simple staining method" that produced overall contrast for X-ray imaging soft tissues similar to how Osmium Tetroxide was also used as a soak for this purpose.
> > 
> > We were interested in using PTA as it doesn't render the soft tissue black like Osmium Tetroxide does, so that there's still a chance we would be able to do further histology work on the tissue. However, we wonder what PTA might do to the fixed yet unprocessed tissue when it is soaked for this imaging purpose, and if it might affect its viability for staining and IHC work.
> > 
> > Thank you,
> > C.
> > 
> >> On Wed, Mar 25, 2015 at 9:11 AM, John Kiernan <jkiernan <@t> uwo.ca> wrote:
> >> PTA can be used on tiny specimens to enhance electron-opacity. For light microscopy, PTA is used in the trichrome methods to enhance differential staining of collagen and cytoplasm, especially in paraffin sections. These uses of PTA are not the same as "soak formalin fixed soft tissue for a few hours before it gets processed". What are you hoping to achieve?
> >> 
> >> John Kiernan
> >> London, Canada
> >> = = =
> >> 
> >>> On 25/03/15, "Cecilia A." <cecystan76 <@t> gmail.com> wrote:
> >>> Hello Histonetters,
> >>> 
> >>> I was wondering if anyone might be able to give some input regarding the
> >>> use of Phosphotungstic acid in which we want to soak formalin fixed soft
> >>> tissue for a few hours before it gets processed. Will subjecting it to PTA
> >>> before processing affect its viability for further histology work (ie, H&E
> >>> staining and IHC)?
> >>> 
> >>> Thank you very much!
> >>> C.
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> > 
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