[Histonet] Tol Blue

[Jennifer MacDonald]

Mast cells granules are metachromatic.  What you see is the expected 
staining reaction.



From:   Kimberly Marshall <Kimberly <@t> animalreferencepathology.com>
To:     "histonet <@t> lists.utsouthwestern.edu" 
<histonet <@t> lists.utsouthwestern.edu>
Date:   03/24/2015 02:20 PM
Subject:        [Histonet] Tol Blue
Sent by:        histonet-bounces <@t> lists.utsouthwestern.edu



?Hello my fellow Histo Techs.  Have a question I just know someone out 
there can answer for me.

In canine tissue, we are having problems with the Tol Blue for mast cell. 
Am experiencing metachromasia, or the mast cells turning purple not blue. 
I have attempted to decrease time, or add time, but its not helping.  My 
pathologist says he has had this issue before.  So question is.  Could it 
be the mast cell in a dog does not stain the same? Is there another stain 
that may work?  Any help will be much appreciated.


Thanks in Advance.

Kimberly Marshall H.T.(ASCP)






Kimberly Marshall H.T.(ASCP)

Histology/Lab Supervisor

Toll Free 1-800-426-2099

Fax 801-584-5104

PO Box 17580

Salt Lake City, Utah 84107

www.animalreferencepathology.com<http://www.animalreferencepathology.com/>



Advancing the art and science of veterinary medicine



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