[Histonet] RE: Masson Trichrome stain

[Mayer,Toysha N]

Justine,

I do not have any metal forceps in the special stains area, due to the reaction that they can cause when staining with silver.  As a rule of thumb, it is just easier to use plastic all the way around.  
The Carson text does not state the use of only plastic forceps, but I would think that maybe they are concerned with a reaction between the Weigert's and the metal.  That would be a stretch.
As for no water before aniline blue, I believe the concentration is very weak and the water may dilute they dye even further.  This would affect the staining results.


Sincerely,

Toysha N. Mayer, D.H.Sc., MBA, HT (ASCP)
Instructor/Education Coordinator
Program in Histotechnology
School of Health Professions
UT M.D. Anderson Cancer Center
713.563-3481






 
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Message: 4
Date: Tue, 10 Mar 2015 00:31:56 -0500
From: John Kiernan <jkiernan <@t> uwo.ca>
Subject: Re: [Histonet] FW: Masson's trichrome stain
To: Linda Margraf <lindamargraf <@t> gmail.com>,
	histonet <@t> lists.utsouthwestern.edu
Cc: justinelanzon <@t> hotmail.com
Message-ID: <7380eaed48941.54fe3b7c <@t> uwo.ca>
Content-Type: text/plain; charset=iso-8859-1

The notion of plastic forceps is new to me. Where did Justine find it? Nothing in any variant of the Masson procedure should be adversely affected by moving slides with stainless steel forceps. Is there a commercial campaign to sell plastic tweezers to Histonetters? 

John Kiernan
= = =
On 08/03/15, Linda Margraf  <lindamargraf <@t> gmail.com> wrote:
> Here is a message from Justine...
> 
> From: Justine Lanzon [mailto:justinelanzon <@t> hotmail.com] 
> <justinelanzon <@t> hotmail.com]>
> Sent: Thursday, March 05, 2015 5:36 AM
> To: lindamargraf <@t> gmail.com
> Subject: Masson's trichrome stain
> 
> 
> Hi,
> 
> I am doing a write up on Masson's trichrome stain however I cannot 
> answer these two questions:
> 
> - Why are plastic forceps used instead of metal ones to hold the 
> stained slide?
> 
> - Why do we not rinse before Alinine blue?
> 
> ?
> 
> Can you please help me?
> 
> ?
> 
> Many Thanks,
> 
> Justine Lanzon
> 
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> 
> 


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