[Histonet] 5-methylcytosine IHC in tissue

[Mariela Chertoff]

Hi

Has anybody experience with 5-metcyt staining in brain tissue? some advice
about the HCl  and Boric acid treatment?
I use 50um free floating sections ferfused with PFA 4%.

Thank you in advance for your help!!

Mariela Chertoff, PhD
Laboratorio de Neuroepigenetica - QB75
 Departamento de Química Biológica
Facultad de Ciencias Exactas y Naturales - UBA
Ciudad Universitaria Pabellón II Piso 4
Ciudad Autónoma de Buenos Aires
C1428EGA - Argentina
Tel: 54 11 4576-3300/09 - Int. 221
email:marielachertoff <@t> gmail.com
marielachertoff <@t> qb.fcen.uba.ar
<mariela.chertoff <@t> uab.cat>