[Histonet] anti-BRAF V600E (VE1) Ventana

[Patrick Laurie]

Spring Bioscience is part of Ventana, Spring had the original clone, and
now so does Ventana.  We use the spring bioscience concentrated antibody on
the Bond III at 1:100 dilution, using the Bond Hi-pH ER2 retrieval for 20
minutes.  I know that Ventana sells the RTU version.  Remember that from
Spring it is RUO, so it is up to you to do the appropriate LDT.  The
Ventana antibody is an IVD. It is also a pretty pricey antibody from either
vendor.

Let me know if you need any further help.

Patrick Laurie(HT)ASCP QIHC

Histology Manager

Celligent Diagnostics, LLC

101 East W.T. Harris Blvd  | Suite 1212 | Charlotte, NC 28262

Work: 704-970-3300  Cell: 704-266-0869

On Tue, Mar 10, 2015 at 1:15 PM, Susan Foreman <sforeman <@t> labpath.com> wrote:

> What vendor are you guys using for antibody anti-BRAF V600E (VE1)?  Spring
> Bioscience or Ventana?  What dilution are you using?  Are you utilizing
> amplification?   Expensive
>
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