[Histonet] RE: Mushrooms for GMS fungus control

[Morken, Timothy]

Try this article...

Acta Cytol. 2003 Nov-Dec;47(6):1043-4.
Alternative, cost-effective fungus-staining method for control slides in cytology and histopathology.
da Silva VD1.
Author information
Abstract
OBJECTIVE:
To develop a cost-effective, reliable and safe method of providing fungal control slides for routine use in pathology laboratories.
STUDY DESIGN:
A set of easily available, low-cost material was tested to obtain fungal colonies on substrate adequate for paraffin-embedded sections or smears.
RESULTS:
Such material as cheese is a simple, inexpensive and practical culture medium for silver-positive fungi. A batch of paraffin blocks can be prepared to maintain a stock of control material in the laboratory.
CONCLUSION:
It is useful to maintain fungal colonies to produce staining control specimens using small pieces of refrigerated cheese to easily produce silver-staining control specimens or smears embedded in paraffin, reducing the risk of accidental exposure to potentially infective pathogens in the laboratory. This method might also be a good alternative for conserving routine surgical specimens, considering the currently decreasing numbers of necropsy and large specimens, particularly from immunosuppressed and infected patients.
PMID: 14674076 [PubMed - indexed for MEDLINE]


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of koellingr <@t> comcast.net
Sent: Sunday, March 08, 2015 4:29 PM
To: Linda Prasad (SCHN)
Cc: Jeffrey Robinson; histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] RE: Mushrooms for GMS fungus control

Apparently there are numerous interesting ways for fungus or bacteria controls to be had from orange peels to hamburger to slim Jim's to hot dogs to strawberries to ????.  Sounds like fun to me.  I'm curious, with the emphasis now on quality control in labs run amok, has anyone passed a rigorous inspection actually showing these as your currently in-use controls?  A PI in research who doesn't want his paper rejected at peer review.  A CAP inspector in clinical labs who is nit-picky reviewing staining controls but might be looking for a phase anything deficiency.  The dot-your-i's and cross-your-t's FDA people who might or might not OK your drug in development.  Really, just curious if anyone with a hammer over your head has said it is perfectly fine to use them. 
Ray, Seattle, WA 

----- Original Message -----

From: "Linda Prasad (SCHN)" <linda.prasad <@t> health.nsw.gov.au>
To: "Jeffrey Robinson" <JRobinson <@t> pathology-associates.com>, histonet <@t> lists.utsouthwestern.edu
Sent: Sunday, March 8, 2015 4:09:02 PM
Subject: [Histonet] RE: Mushrooms for GMS fungus control 

I used strawberries for a fungal control. Worked really good. 

Linda Prasad | Senior Scientist | Histopathology
t: (02) 9845 3306 | f: (02) 9845 3318 | e: linda.prasad <@t> health.nsw.gov.au | w: www.schn.health.nsw.gov.au 

Cnr Hawkesbury Road and Hainsworth Street, Westmead, NSW Australia Locked Bag 4001, Westmead 2145, NSW Australia 

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jeffrey Robinson
Sent: Saturday, 7 March 2015 4:16 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Mushrooms for GMS fungus control 

How about mushrooms?  Has anyone had any success using mushrooms as a GMS fungus control? 

Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA 


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