[Histonet] Considerations about Warthin-Starry principle
Gudrun Lang
gu.lang <@t> gmx.at
Sun Mar 8 01:38:56 CST 2015
Dear histonetters,
I came across a question regarding the Warthin-Starry principle. Are
bacterial catalase or oxidase the cause for developement of silver-deposits?
The usual explanation is, that bacteria are able to bind silver onto their
surface, that is further reduced by hydrochinon to visible silver-deposits.
But why?
Some considerations:
The pH must be held at pH4 with citric acid in the WS developement-solution.
(Is this the pH-optimum for the bacterial enzymes?)
IHC shows that endogenous peroxidase survives the embedding-process. (Is
this also true for the bacterial enzymes?)
Spirochetes (and others) are catalase and oxidase positive.
Silver-In situ hybdridisation technique shows, that peroxidase with H2O2 can
be used to reduce Silveracetate for silver-deposits.
Is there a possible relationship between the bacterial enzymes and the WS
principle?
Or am I totally on an erroneous way? What do you think?
thanks for any input.
Gudrun Lang
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