[Histonet] Masson trichrome and H and E
Pablo Sanchez-Quinteiro
pablo.sanchez <@t> usc.es
Wed Mar 4 02:32:43 CST 2015
>>At 19:19 03/03/2015, you wrote:
>>Hi!
>>For lacking Eosin-staining check the pH of the solution. It should
be 4-5.
><With some drops of acetic acid you may recover the staining-action.
>>Gudrun
>>= = =
Thanks for the tip! Nice to know it... after centuries doing stainings
:-)
Pablo
On 03/03/15, Emily Brown <talulahgosh <@t> gmail.com> wrote:
> Hello!
>
> Another question, I did H and E and there is no eosin staining. I
> think the reagents are pretty old, so I thought that might be a
> problem. Also because my lab is cheap, they were reusing the xylenes
> and EtOH for both rehydrating and rehydrating. I told my boss this
is
> probably not a good idea as the end steps will have stain in them.
And
> I also think this is why it didn't work! The EtOH is also really old
> so who know is the 100% is actually even close to 100% any more. I'm
> buying new reagents, but if you guys think anything else would help,
let
me know.
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