[Histonet] Masson trichrome and H and E

[Pablo Sanchez-Quinteiro]

   >>At 19:19 03/03/2015, you wrote:
   >>Hi!
   >>For  lacking  Eosin-staining check the pH of the solution. It should
   be 4-5.
   ><With some drops of acetic acid you may recover the staining-action.
   >>Gudrun
   >>= = =
   Thanks for the tip! Nice to know it... after centuries doing stainings
   :-)
   Pablo
   On 03/03/15, Emily Brown  <talulahgosh <@t> gmail.com> wrote:
   > Hello!
   >
   > Another question, I did H and E and there is no eosin staining. I
   > think the reagents are pretty old, so I thought that might be a
   > problem. Also because my lab is cheap, they were reusing the xylenes
   >  and  EtOH for both rehydrating and rehydrating. I told my boss this
   is
   >  probably  not a good idea as the end steps will have stain in them.
   And
   > I also think this is why it didn't work! The EtOH is also really old
   > so who know is the 100% is actually even close to 100% any more. I'm
   > buying new reagents, but if you guys think anything else would help,
   let
   me know.