AW: [Histonet] Masson trichrome and H and E

[Rene J Buesa]

Emely:
To your initial e-mail the kit is so expensive because it incorporates a salary component for the preparation of the solutions + any and all enormous profit margin the manufacturer wants to add.I never/ever used kits, always used solutions prepared "from scratch" in my lab and the savings were enormous.As to preferred dry dyes my best preferred are Merk (Darmstad), Harleco and Sigma.René J.  

     On Tuesday, March 3, 2015 1:19 PM, Gudrun Lang <gu.lang <@t> gmx.at> wrote:
   

 Hi!
For lacking Eosin-staining check the pH of the solution. It should be 4-5.
With some drops of acetic acid you may recover the staining-action.
Gudrun
= = =
On 03/03/15, Emily Brown  <talulahgosh <@t> gmail.com> wrote:
> Hello!
> 
> I am doing Masson trichrome manually (not with a machine) and I just 
> found out the kit is $800!! I was thinking of buying the ingredients 
> separately, but why the hell is it so expensive???
> Also, the people I was going to borrow their reagents from said their 
> aniline blue is not very good and I wanted to replace it. I only need 
> about 250ml, what brand do you guys prefer?
> l know I can google this, but I want to know what you guys like and 
> what works best. This is for mouse kidney paraffin sections, 4 to 5
microns.
> Another question, I did H and E and there is no eosin staining. I 
> think the reagents are pretty old, so I thought that might be a 
> problem. Also because my lab is cheap, they were reusing the xylenes 
> and EtOH for both rehydrating and rehydrating. I told my boss this is 
> probably not a good idea as the end steps will have stain in them. And 
> I also think this is why it didn't work! The EtOH is also really old 
> so who know is the 100% is actually even close to 100% any more. I'm 
> buying new reagents, but if you guys think anything else would help, let
me know.
> 
> Also, shoutout to Ann, I know you're reading these!! Join the list!!
> 
> Emily
> 
> 
> "By bitching and bitching and bitching, they could exhaust the drama 
> of their own horror stories. Grow bored. Only then could they accept a 
> new story for their lives. Move forward."
> 
> -Chuck Palahniuk, "Haunted"
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