AW: [Histonet] Masson trichrome and H and E

[Gudrun Lang]

Hi!
For lacking Eosin-staining check the pH of the solution. It should be 4-5.
With some drops of acetic acid you may recover the staining-action.
Gudrun
= = =
On 03/03/15, Emily Brown  <talulahgosh <@t> gmail.com> wrote:
> Hello!
> 
> I am doing Masson trichrome manually (not with a machine) and I just 
> found out the kit is $800!! I was thinking of buying the ingredients 
> separately, but why the hell is it so expensive???
> Also, the people I was going to borrow their reagents from said their 
> aniline blue is not very good and I wanted to replace it. I only need 
> about 250ml, what brand do you guys prefer?
> l know I can google this, but I want to know what you guys like and 
> what works best. This is for mouse kidney paraffin sections, 4 to 5
microns.
> Another question, I did H and E and there is no eosin staining. I 
> think the reagents are pretty old, so I thought that might be a 
> problem. Also because my lab is cheap, they were reusing the xylenes 
> and EtOH for both rehydrating and rehydrating. I told my boss this is 
> probably not a good idea as the end steps will have stain in them. And 
> I also think this is why it didn't work! The EtOH is also really old 
> so who know is the 100% is actually even close to 100% any more. I'm 
> buying new reagents, but if you guys think anything else would help, let
me know.
> 
> Also, shoutout to Ann, I know you're reading these!! Join the list!!
> 
> Emily
> 
> 
> "By bitching and bitching and bitching, they could exhaust the drama 
> of their own horror stories. Grow bored. Only then could they accept a 
> new story for their lives. Move forward."
> 
> -Chuck Palahniuk, "Haunted"
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