[Histonet] Suggestions for staining ground substances in the Heart

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Tue Jun 30 18:46:36 CDT 2015

If the ground substance and mucin are not staining, it would be a problem with the alcian blue solution.

Microscopically check the slides after the alcian blue step - if no or weak staining then:
1.	Is the alcian blue at pH 2.5 or is it pH1 - (goblet cells in stomach and ground substance rarely stain at pH 1)?
2.	Is the alcian blue dye of good quality. There are several out there - use Certified dyes.

Hopefully this helps

Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist, the Children's Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 

-----Original Message-----
From: Wolfe, Christina [mailto:christina.wolfe at bms.com] 
Sent: Wednesday, 1 July 2015 1:15 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Suggestions for staining ground substances in the Heart

Hi all,
We are interested in staining ground substances in the heart.  Are there other stains that will work beside the Pentachrome?  We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain.  In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining.  We have tried sections cut at 4 and 6 microns.  Any thoughts/suggestions?


Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC Drug Safety Evaluation/Bristol-Myers Squibb Pathology Dept.

This message (including any attachments) may contain confidential, proprietary, privileged and/or private information. The information is intended to be for the use of the individual or entity designated above. If you are not the intended recipient of this message, please notify the sender immediately, and delete the message and any attachments. Any disclosure, reproduction, distribution or other use of this message or any attachments by an individual or entity other than the intended recipient is prohibited.
Histonet mailing list
Histonet at lists.utsouthwestern.edu

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Sydney Children's Hospitals Network.

This note also confirms that this email message has been virus scanned and although no computer viruses were detected, The Sydney Childrens Hospital's Network accepts no liability for any consequential damage resulting from email containing computer viruses.

More information about the Histonet mailing list