[Histonet] Suggestions for staining ground substances in the Heart

[Wolfe, Christina]

Hi all,
We are interested in staining ground substances in the heart.  Are there other stains that will work beside the Pentachrome?  We have tried the Movat's pentachrome (commercial kit) and are able to demonstrate ground substance in the bone with the alcian blue part of this stain.  In our hands the goblet cells of the gut and the ground substance in the heart are devoid of staining.  We have tried sections cut at 4 and 6 microns.  Any thoughts/suggestions?

Kristie

Christina Wolfe, BSHA, MLT (ASCP), HT, QIHC
Drug Safety Evaluation/Bristol-Myers Squibb
Pathology Dept.
812-307-2093


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