[Histonet] pre-floating tissue sections
Galina Deyneko
galinadeyneko at yahoo.com
Mon Jun 8 09:10:04 CDT 2015
Hi Colleagues, I also use this technique with intermediate bath with cold DI water and like it. also i breath on the surface of the block befor make one section, this helps making the section more smooth. Galina DeynekoNovartis, Cambridge, MA 617-871-7613 w
From: "histonet-request at lists.utsouthwestern.edu" <histonet-request at lists.utsouthwestern.edu>
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Subject: Histonet Digest, Vol 139, Issue 7
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Today's Topics:
1. Re: Paraffin block disposal (Aimee Tolentino)
2. Re: Paraffin block disposal (Cooper, Brian)
3. Re: tissue fixation-formaldehyde concentrations (Hobbs, Carl)
4. Re: Paraffin block disposal (Brendal Finlay)
5. Re: Pre-floating tissue sections in dilute alcohol
(tjfinney2010 at gmail.com)
6. Re: Decalcification of bone marrows (Bob Richmond)
7. Re: Pre-floating tissue sections in dilute alcohol (Joana Moreira)
8. Re: Pre-floating tissue sections in dilute alcohol (Gudrun Lang)
----------------------------------------------------------------------
Message: 1
Date: Sat, 6 Jun 2015 10:24:42 -0700
From: Aimee Tolentino <a.tolentino82 at gmail.com>
To: "Arbaugh, Roberta" <rarbaugh at csdermatology.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Paraffin block disposal
Message-ID: <1F36ABA5-452B-4556-8D1E-E5D09FDB22FC at gmail.com>
Content-Type: text/plain; charset=us-ascii
That's a good question. I'd like to know the answer myself to that. :)
Sent from my iPhone
> On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta <rarbaugh at csdermatology.com> wrote:
>
> Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them?
>
> DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you.
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Message: 2
Date: Sat, 6 Jun 2015 18:34:17 +0000
From: "Cooper, Brian" <bcooper at chla.usc.edu>
To: "a.tolentino82 at gmail.com" <a.tolentino82 at gmail.com>
Cc: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Paraffin block disposal
Message-ID:
<C12647AD4408834C8AB48FB0389C63E3E424398C at CHLAEXMBH01.LA.AD.CHLA.ORG>
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Hey Aimee,
This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, "What about CJD?" and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . .
Thanks,
Brian Cooper, HT (ASCP)
Supervisor, Histology
Children's Hospital, Los Angeles
Sent from my Galaxy S5, so please forgive any weird typos . . .
-----Original Message-----
From: Aimee Tolentino [a.tolentino82 at gmail.com]
Received: Saturday, 06 Jun 2015, 10:25AM
To: Arbaugh, Roberta [rarbaugh at csdermatology.com]
CC: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu]
Subject: Re: [Histonet] Paraffin block disposal
That's a good question. I'd like to know the answer myself to that. :)
Sent from my iPhone
> On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta <rarbaugh at csdermatology.com> wrote:
>
> Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them?
>
> DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you.
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Message: 3
Date: Sat, 6 Jun 2015 19:08:47 +0000
From: "Hobbs, Carl" <carl.hobbs at kcl.ac.uk>
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] tissue fixation-formaldehyde concentrations
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I have to agree with the student, John.
Sure, he is coming from ignorance ( not a bad situation: naivety is not a fault... we all are/were there at some point;-)
Sure...I agree with you re the using of the word Paraformaldehyde as a fixative....
I often "sigh" when used.
However, differential fixation ( zonal fixation) has always been an issue.
We often see the zonal effects of this?
Particularly in lymph nodes ( parenchymatous tissues)
Obviously, when immersion fixing.
Most often ...NOT with agitation.
Respectfully,
Carl
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge?
Kings College London
London
SE1 1UL
?
020 7848 6813
------------------------------
Message: 4
Date: Sat, 06 Jun 2015 15:29:18 -0500
From: "Brendal Finlay" <brendal.finlay at medicalcenterclinic.com>
To: a.tolentino82 at gmail.com, Cooper, Brian <bcooper at chla.usc.edu>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Paraffin block disposal
Message-ID: <ba90f95c9f538f0650d9e98cfde75283 at medicalcenterclinic.com>
Content-Type: text/plain; charset=UTF-8
I agree with Brian, but we dispose of? blocks by treating them as regulated biohazard waste. We also?blocks them longer than 2 years.?The CLIA regulation states keeping them for a minimum of 2 years.?Outside facilities frequently request unstained slides or blocks?on?cases that are more than 2 years old.?Also, some patients require treatment for conditions for many years after the specimen is taken. If storage is not an issue,?keeping blocks?10 years (CAP requirements) is reasonable.
?
Brendal C. Finlay, HT (ASCP)
Senior Histologist
Medical Center Clinic, P.A
8333 North Davis Highway
Pensacola, FL 32514
Phone 850.474.8581
Fax 850.474.8584?
-----Original Message-----
From: "Cooper, Brian" <bcooper at chla.usc.edu>
To: a.tolentino82 at gmail.com
Cc: histonet at lists.utsouthwestern.edu
Date: 06/06/2015 13:34
Subject: Re: [Histonet] Paraffin block disposal
Hey Aimee,
This has been discussed several times on Histonet. It sounds like it depends on the institution. Since they're FFPE, pathogens are not a concern. I didn't reply to all because someone will shout out, "What about CJD?" and then I would have to punch them. They should be able to go into the regular trash though, since there is nothing that anyone can catch from them. Here, just like Genzyme, we are told to dispose of them as regulated, biohazard waste. You would have PHI concerns if the patient's name is on them, so they'll need to be identified first . . .
Thanks,
Brian Cooper, HT (ASCP)
Supervisor, Histology
Children's Hospital, Los Angeles
Sent from my Galaxy S5, so please forgive any weird typos . . .
-----Original Message-----
From: Aimee Tolentino [a.tolentino82 at gmail.com]
Received: Saturday, 06 Jun 2015, 10:25AM
To: Arbaugh, Roberta [rarbaugh at csdermatology.com]
CC: histonet at lists.utsouthwestern.edu [histonet at lists.utsouthwestern.edu]
Subject: Re: [Histonet] Paraffin block disposal
That's a good question. I'd like to know the answer myself to that. :)
Sent from my iPhone
> On Jun 5, 2015, at 12:54 PM, Arbaugh, Roberta <rarbaugh at csdermatology.com> wrote:
>
> Per CLIA we only need to keep paraffin blocks two years. What is the proper way to dispose of them?
>
> DISCLAIMER: The information in this message is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, or distribution of the message, or any action or omission taken by you in reliance on it, is prohibited and may be unlawful. Please immediately contact the sender if you have received this message in error. Thank you.
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Message: 5
Date: Sat, 06 Jun 2015 15:09:30 -0700 (PDT)
From: tjfinney2010 at gmail.com <tjfinney2010 at gmail.com>
To: garreyf at gmail.com <garreyf at gmail.com>,
histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol
Message-ID: <000f425a.2883754973a8f7e7 at gmail.com>
Content-Type: text/plain; charset="utf-8"
Yes I use it for brain.
Happy Connecting. Sent from my Sprint Phone.
------ Original message------
From: Garrey Faller
Date: Sat, Jun 6, 2015 10:48 AM
To: histonet at lists.utsouthwestern.edu;
Subject:[Histonet] Pre-floating tissue sections in dilute alcohol
Hi everyone,
I just became aware of this technique last week, and it seems to work great.
I did a quick google search and found this quick reference.
http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf
Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath?
Its an extra step and introduces an extra chance to introduce floaters.
But, the quality seems to be improved.
Any thoughts?
Thanks in advance.
Garrey Faller
Pathologist
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Message: 6
Date: Sat, 6 Jun 2015 19:19:53 -0400
From: Bob Richmond <rsrichmond at gmail.com>
To: "Histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Decalcification of bone marrows
Message-ID:
<CAOKsRH66N1cx7yvzARps=fQx436sL03rNCL_T=KM=Z63eux+1g at mail.gmail.com>
Content-Type: text/plain; charset=UTF-8
Adrienne (where?) asks: "I have a really quick question: about how long
does it take to decal a bone marrow biopsy?" to which Jessica (where? -
apparently in the US though) replies "It all depends on what you use for
decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit
more time."
To decalcify a Jamshidi needle bone marrow biopsy specimen in one of the
ordinary commercial decalcifiers (usually hydrochloric acid, all deep dark
trade secrets) takes about two hours.
Nitric acid is NOT an acceptable decalcifier today, since it destroys
immunoreactivity. I used to use it for most decalcification in the days
before immunohistochemistry, but remember I've been practicing pathology
for more than fifty years.
Successful processing of bone marrow biopsy specimens requires cooperation
among hematologist-oncologists, pathologists, and histotechnologists.
(Dream on!) Practically speaking, you have to set a deadline - if your
specimen arrives after 2 PM (for example) it doesn't get processed until
tomorrow.
Once again - Decal is the registered trademark of Decal Chemical
Corporation's proprietary decalcifier. It is not a generic word for
decalcifiers. (No, I don't work for them.)
Bob Richmond
Samurai Pathologist
Maryville, TN
------------------------------
Message: 7
Date: Sun, 7 Jun 2015 04:17:37 +0000
From: Joana Moreira <jmoreira at sidra.org>
To: "histonet at lists.utsouthwestern.edu"
<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol
Message-ID:
<A478EF23B3AAD44CAD6804356D5676BB1053071B at MV3WEXMX03PRV.smrc.sidra.org>
Content-Type: text/plain; charset="iso-8859-1"
I Garrey,
I came across floating sections in dilute alcohol back in 2005 when I started working in the UK and have been using it ever since.
I agree it introduces one more step and wouldn't use for every single section - there's no need. But I do find it very helpful with certain blocks/tissues - especially the very 'wrinkly' ones.
When I started cutting during my training, we used other technique to avoid wrinkles (and waste of tissue sections) and produce 'perfect' sections. We used to have a small cold water bath to float and pick up the sections before transferring to the temperature controlled water bath.
Joana
Joana Moreira
Supervisor - Anatomical Pathology
Department of Pathology
Sidra Medical & Research Center
PO Box 26999 | Doha, Qatar
Direct Line +974-4404-2036
jmoreira at sidra.org | www.sidra.org
Message: 12
Date: Sat, 6 Jun 2015 10:29:03 -0400
From: Garrey Faller <garreyf at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Pre-floating tissue sections in dilute alcohol
Message-ID: <A7285EF9-FA0F-4121-AA4C-78DFA5F1CE4E at gmail.com>
Content-Type: text/plain; charset=us-ascii
Hi everyone,
I just became aware of this technique last week, and it seems to work great.
I did a quick google search and found this quick reference.
http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf <http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf>
Anyone out there float their sections in dilute ethyl alcohol before transferring to the water bath?
Its an extra step and introduces an extra chance to introduce floaters.
But, the quality seems to be improved.
Any thoughts?
Thanks in advance.
Garrey Faller
Pathologist
------------------------------
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Message: 8
Date: Sun, 7 Jun 2015 12:38:43 +0200
From: "Gudrun Lang" <gu.lang at gmx.at>
To: "'Garrey Faller'" <garreyf at gmail.com>
Cc: <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Pre-floating tissue sections in dilute alcohol
Message-ID: <000001d0a10e$20f95690$62ec03b0$@gmx.at>
Content-Type: text/plain; charset="iso-8859-1"
Some of our histotechs use just cold tapwater for wrinkled sections before
transferring them into the warm waterbath. I don't know, if the added
ethanol makes any difference (maybe reducing surface tension).
I would offer the possibility of using the additional waterbath, but let the
histotechs decide, if it is necessary to use it.
Gudrun Lang
Leading histotech, Linz Austria
-----Urspr?ngliche Nachricht-----
Von: Garrey Faller [mailto:garreyf at gmail.com]
Gesendet: Samstag, 06. Juni 2015 16:29
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Pre-floating tissue sections in dilute alcohol
Hi everyone,
I just became aware of this technique last week, and it seems to work great.
I did a quick google search and found this quick reference.
http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf
<http://www.scielo.cl/pdf/ijmorphol/v30n1/art07.pdf>
Anyone out there float their sections in dilute ethyl alcohol before
transferring to the water bath?
Its an extra step and introduces an extra chance to introduce floaters.
But, the quality seems to be improved.
Any thoughts?
Thanks in advance.
Garrey Faller
Pathologist
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Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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