[Histonet] PAS stain

Tony Henwood (SCHN) tony.henwood at health.nsw.gov.au
Tue Jun 2 16:02:46 CDT 2015

Hi Mica,

Most haematoxylins will do eg Harris or Mayer's.
I would recommend a light counterstain (it is a counterstain, not the main stain).
Differentiate if too strong.

Also periodic acid treatment increases nuclear affinity for Hx so shorten the counterstain time.

From: Mica [faith14913 at aol.com]
Sent: Tuesday, 2 June 2015 1:02 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] PAS stain

Just a question...what kind of hematoxylin do you use as a counter stain for your PAS stain and do you use a differentiator?

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