[Histonet] PAS-diastase staining

[Julio Benavides]

Yes, they have, and it is a mixture of alfa and beta amylase, which I  
think should be better.

Thanks a lot Bernice

Julio



Bernice Frederick <b-frederick at northwestern.edu> escribió:

> Fisher should gave it. I know they have diastase of malt....
>
> Bernice Frederick HTL (ASCP)
> Senior Research Tech
> Pathology Core Facility
> Robert. H. Lurie Cancer Center
> Northwestern University
> 710 N Fairbanks Court
> Olson 8-421
> Chicago,IL 60611
> 312-503-3723
> b-frederick at northwestern.edu
>
>
> -----Original Message-----
> From: Julio Benavides [mailto:j.benavides at eae.csic.es]
> Sent: Monday, June 01, 2015 6:29 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: Re: [Histonet] PAS-diastase staining
>
> Hi,
>
> Sigma has discontinued selling type VIII-alfa amylase from barley  
> (catalogue ref A2771). Anyone knows any other commercial source of  
> this enzyme or if the other amylase (other types of alfa amylase or  
> beta amylase) work for PAS-D?
>
> Thanks
>
>
> Julio
>
> Bea DeBrosse-Serra <BDeBrosse-Serra at isisph.com> escribió:
>
>> Hi Julio,
>>
>> Here is our procedure. It is very straight forward.
>>
>> Beatrice
>>
>> Beatrice DeBrosse-Serra HT(ASCP)QIHC
>> Isis Pharmaceuticals
>> Antisense Drug Discovery
>> 2855 Gazelle Ct.
>> Carlsbad, CA 92010
>> 760-603-2371
>>
>>
>>
>>
>> -----Original Message-----
>> From: Julio Benavides [mailto:j.benavides at eae.csic.es]
>> Sent: Friday, May 29, 2015 6:35 AM
>> To: histonet at lists.utsouthwestern.edu
>> Subject: [Histonet] PAS-diastase staining
>>
>> Hi there,
>>
>> I am trying to do a PAA-diastase in formalin-fixed embedded samples
>> (ovine liver). I would be most grateful if someone could give me a
>> hand in:
>>
>> -Protocol (enzyme concentration, time of digestion...) for diastase
>> digestion (I am assuming that PAS afterwards is the normal protocol
>> for PAS) -A commercial source for diastase working in these samples.
>> Is the porcine amylase from sigma any good? cheaper options?
>>
>> As always,
>>
>> thank you very much for all your comments
>>
>> Cheers
>>
>> Julio
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet at lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet