[Histonet] PAS-diastase staining

[Julio Benavides]

Hi,

Sigma has discontinued selling type VIII-alfa amylase from barley  
(catalogue ref A2771). Anyone knows any other commercial source of  
this enzyme or if the other amylase (other types of alfa amylase or  
beta amylase) work for PAS-D?

Thanks


Julio

Bea DeBrosse-Serra <BDeBrosse-Serra at isisph.com> escribió:

> Hi Julio,
>
> Here is our procedure. It is very straight forward.
>
> Beatrice
>
> Beatrice DeBrosse-Serra HT(ASCP)QIHC
> Isis Pharmaceuticals
> Antisense Drug Discovery
> 2855 Gazelle Ct.
> Carlsbad, CA 92010
> 760-603-2371
>
>
>
>
> -----Original Message-----
> From: Julio Benavides [mailto:j.benavides at eae.csic.es]
> Sent: Friday, May 29, 2015 6:35 AM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] PAS-diastase staining
>
> Hi there,
>
> I am trying to do a PAA-diastase in formalin-fixed embedded samples  
> (ovine liver). I would be most grateful if someone could give me a  
> hand in:
>
> -Protocol (enzyme concentration, time of digestion...) for diastase  
> digestion (I am assuming that PAS afterwards is the normal protocol  
> for PAS) -A commercial source for diastase working in these samples.  
> Is the porcine amylase from sigma any good? cheaper options?
>
> As always,
>
> thank you very much for all your comments
>
> Cheers
>
> Julio
>
>
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