[Histonet] Ventana H.Pylori antibody
MWhite at mhs.net
Fri Jul 31 12:09:00 CDT 2015
We also are having the same problem with the Ventana H.Pylori antibody on our Ultras, I have tried the CellMarque product in the past and as mentioned still not ideal. Will try the Biocare product and see if the results improve for us also.
Marcia M White
Memorial Regional Hospital
MWhite at mhs.net
From: Jeffrey Robinson [mailto:JRobinson at pathology-associates.com]
Sent: Thursday, July 30, 2015 1:06 PM
To: Cates, Julia; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana H.Pylori antibody
Hi Julia- I have had a lot of experience trying to get a clean H. pylori. I don't recall using the Ventana H. pylori but I was using the CellMarque H. pylori for years. It would often have a lot of background as you describe. I run Ventana Benchmarks (XT and Ultra) and a Leica Bond. The slides I would run on the Benchmarks were usually cleaner than those run on the Bond but still not as clean as I would like. I finally switched over to BioCare's H. pylori after many complaints from my pathologists. It has been much cleaner on all of my IHC instruments. Actually, the ones I run on the Ultra are the cleanest I have ever seen. The slides run on the Bond may still have a little background but it is still much cleaner than the ones I used to run with the CellMarque antibody. One of my pathologists who used to complain about the excessive background now calls it his "go to" stain and is very happy with how crisp it is. The organisms stand out very nicely. BioCare will send you a free sample if you want to try it.
Jeff Robinson, Senior Histotechnologist, Sierra Pathology Lab, Clovis, CA.
From: Cates, Julia via Histonet [mailto:histonet at lists.utsouthwestern.edu]
Sent: Thursday, July 30, 2015 8:30 AM
To: histonet-bounces at lists.utsouthwestern.edu; histonet at lists.utsouthwestern.edu
Subject: [Histonet] Ventana H.Pylori antibody
Good day Histonet,
I have seen in the past conversations regarding Ventana's H.Pylori antibody and how dirty looking the stain can be. We have been using this antibody for several years now and have never really been happy with the quality. We have tried the recommended methods to "clean up" the stain and still we run into repeating the stain and/or complaints from the pathologists. We are considering using a different vendor but my concern is that my efforts will be a lateral move. Is anyone using a product that produces a clean stain or is this something inherent to this antibody? Are the pathologists happy with it and not just tolerating it?
Thank you for your suggestions,
Julia Cates, HT(ASCP)cm
Pathology Coordinator, Pathology
Florida Hospital Waterman
(352) 253-3333 ext.4346 | Fax: (352) 253-3592
Confidentiality Statement: This email message, including any attachments, is for the sole use of the intended recipient and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, please contact the sender by reply to this email and delete the original and all copies of this email.
Histonet mailing list
Histonet at lists.utsouthwestern.edu
This email and attachments may contain PHI that is privileged and confidential and is not intended for any unauthorized person. If you, the reader, are not the intended recipient you are hereby notified that any dissemination, distribution or copying of this communication is strictly prohibited. Do not read the email but instead reply to the sender and destroy the message and any attachments. Thank you.
CONFIDENTIALITY NOTICE: DO NOT FORWARD THIS MESSAGE TO OTHERS WITHOUT PERMISSION OF THE SENDER.
This e-mail, including any attachments, may contain confidential or privileged material that is exempt from disclosure under applicable law. Any unauthorized review, use, disclosure, dissemination, copying, or taking any action in reliance on its contents is prohibited. If you have any reason to believe this e-mail was not intended for you, please delete the e-mail and any attachments, and notify the sender immediately.
More information about the Histonet