[Histonet] NetWell inserts and IHC with TUNEL stain

[Emily Dewar]

Hello,

I will be performing a number of immunos within the next couple of months,
and have been wondering what the best way to do so might be with no tissue
damage during the process. Does anyone know whether using NetWell inserts
for immunos to transfer tissue affects morphology? The tissue will be
placed into the insert, and submersed in solution within a well plate to be
incubated on a shaker. The problem is that contact with the NetWell insert
could damage the tissue, not only with the rocking, but with transfer from
one solution to another.

I am under the impression that with TUNEL staining, is often difficult to
differentiate whether the damage in cells caused by handling/extraction, or
from the treatment itself. While not impossible, I will not have the time
to develop or perform such a procedure.

If anyone has any knowledge or insight, it would be greatly appreciated.

Thank you for your time,

Emily Dewar
Laboratory Technician