[Histonet] axolotl lymphatics

John Kiernan jkiernan at uwo.ca
Tue Jul 28 11:06:29 CDT 2015

Instead of an antibody, you might consider enzyme activity histochemistry, which is much less expensive. 
Demonstration of 5-nucleotidase activity in the presence of levamisole detects lymphatic endothelium. Sections can also be stained for alkaline phosphatase activity in the endothelium of blood vessels. Here are a few references.

Kato, S., Yasunaga, A. and Uchida, U. (1991). Enzyme-histochemical method for identification of lymphatic capillaries. Lymphology 24:125-129.
Ohkuma, M. (1994). Simultaneous double staining for the blood and lymphatic capillary. Lymphology 27, Suppl:106-107.
Okada, E. (1994). An improved enzyme-histochemical method for identification of lymphatic capillaries on paraffin sections. Lymphology 27, Suppl:732-735.
Ji, R.C. and Kato, S. (2003). Lymphatic network and lymphangiogenesis in the gastric wall. Journal of Histochemistry and Cytochemistry 51:331-338.

Needless to say, none of these relate to amphibian tissues!

John Kiernan
Anatomy, UWO, London, Canada
= = =
On 26/07/15, Jason Palmer via Histonet  <histonet at lists.utsouthwestern.edu> wrote:
> Hi all, 
> I need to find an antibody that will label lymphatic endothelial cells in the axolotl. Does anybody have any experience or ideas? I have tried a couple of our anti-mouse and anti-human Abs for podoplanin and LYVE-1 but no cross-reactivity so far. I have no experience with staining of non-mammalian tissues - maybe an anti-frog Ab would cross react? Does anyone have experience with other amphibians? 
> Thanks, 
> Jason 
> -- 
> Jason Palmer 
> Histology Laboratory Coordinator 
> O'Brien Institute / St Vincent's Institute 
> 42 Fitzroy St, Fitzroy Victoria 3065 
> Australia 
> tel +61 3 9288 4045 
> fax +61 3 9416 0926 
> email: jpalmer at svi.edu.au 
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