[Histonet] understanding reagents in decalcifier; making it in-house

[Rene J Buesa]

If you can make the decalcifier with that recipe, you will be OK. You can even calculate costs and probably it will be cheaper than buying it from Shandon.René 


     On Wednesday, July 22, 2015 4:36 PM, M.O. via Histonet <histonet at lists.utsouthwestern.edu> wrote:
   

 Hello Histonet,

The supplier for our decalcifier, TBD-2 from Shandon, is having issues with
getting the product out and we will not be receiving it for at least
another month.  Our samples are piling up and I don't know what I should
do, but maybe I can make the decalcifier in-house.  I am wondering if I can
make my own based on the reagents they listed and their percentages and if
certain reagents are not actually necessary.

The samples we typically decalcify are mouse knees (decal time = 2 days),
mouse spines (3 days), human bone slabs about 7mm in thickness (7-12
days).  Fixation is in zinc buffered formalin, then decalcification, then
70% EtOH.  Our choice to use TBD-2 is due to the gentle decalcification for
IHC and we get GREAT results.

Composition of Shandon TBD-2 Decalcifier:
Component                Weight %
    Water                      77-80
 Formic acid                21-23
    Fluorad                      >1
 Sodium citrate              >1
Polyvinyl pyrrolidone      >1


If you have any input on what reagents I should use and the percentages for
making a decalcifier myself, it would be much appreciated!

Thank you for you help,
Merissa
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