[Histonet] Ventana LCS (Liquid Cover Slip)/ non-specific staining

[Burnett, Brandy]

Just wanted to thank everyone for all the helpful input in regards to the Ventana BenchMark Ultra.
We are seeing some non-specific background staining on our slides. It is not so much an issue for
our nuclear/membranous stains, however for our cytoplasmic stains, it could be interpreted as a false
positive. We bake our slides offline at 65 degrees Celsius for 30 minutes, then Depar online (EZ Prep) at 72 degrees
Celsius. Any feedback would be greatly appreciated!

Thanks again,

Brandy Burnett
Histotechnologist, QIHC(ASCP)
CCH Pathology/Histology
bburnett at capecodhealth.org


        Expert physicians. Quality hospitals. Superior care. 

-----Original Message-----
From: Burnett, Brandy [mailto:bburnett at CapeCodHealth.org] 
Sent: Thursday, July 02, 2015 8:32 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Ventana LCS (Liquid Cover Slip)

Anyone out there having any issues with the Ventana LCS?
When you are cover slipping are you seeing any water/residual LCS on the slides?
I was wondering if we should dry our slides before cover slipping instead of running them down? Any information would be greatly appreciated!

Brandy Burnett
Histotechnologist, QIHC(ASCP)
CCH Pathology/Histology
bburnett at CapeCodHealth.org<mailto:bburnett at CapeCodHealth.org>


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