[Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
Nancy.Stedman at buschgardens.com
Mon Jul 13 14:12:21 CDT 2015
We are using Excell Plus made by American Master Tech. It is a combination of alcohol, ethylene glycol, and glyoxal. It does not fix quite as fast as formalin but fixes just as well as long as tissue samples are small and not too fatty. We work in veterinary pathology and mostly with necropsy tissues, and try to keep everything 5 to 10 mm maximum thickness that goes into Excell Plus. It definitely won't fix an entire intact brain no matter how long you let it sit so we book the brains into thin slices 24 hours after collection. We have done some limited IHC and DNA extraction from Excell Plus fixed tissue and have not had any issues. We tried another low tox fixative too - Statlab's GTF formalin substitute, which is glyoxal - and it did not fix as well as Excell Plus. GTF fixed CNS tissues especially had a lot of artifact like shrunken neurons.
Hope this is helpful. I would also be curious to know how many labs use formalin substitutes because it seems like they have not caught on much (or at all) in the veterinary world.
Nancy L. Stedman DVM PhD Dipl ACVP
Busch Gardens Tampa
nancy.stedman at buschgardens.com
From: Adesupo, Adesuyi (Banjo) [mailto:abadesuyi at nrh-ok.com]
Sent: Monday, July 13, 2015 12:52 PM
To: 'histonet at lists.utsouthwestern.edu'
Subject: [Histonet] National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories
I read this article (National Academy of Sciences Confirms That Formaldehyde Can Cause Cancer in a Finding That Has Implications for Anatomic Pathology and Histology Laboratories) this morning.
I wanted to know whether some of you guys out there are using Formaldehyde substitute.
Banjo Adesuyi, BMLS, HT (ASCP) HTL, QIHC, QLS
Norman Regional Health System,
Norman, OK 73071.
Tel: 405- 307- 1145
abadesuyi at nrh-ok.com<mailto:abadesuyi at nrh-ok.com>
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