[Histonet] Ventana antibody - H pylori

[Dyer, Terry]

Happy Friday1
We are experiencing the same problem.  Just had a site visit from Ventana and they suggested using an antibody dilution buffer.   Use an option kit filled with  Ventana Product ADB250 (antibody dilution buffer).  Check options in protocol editing and 8 minutes, suggested time.  Have not tried it but inted to.  Good luck.

-----Original Message-----
From: Susan Foreman [mailto:sforeman at labpath.com] 
Sent: Friday, July 10, 2015 5:08 AM
To: 'Sebree Linda A'; 'Cartun, Richard'; 'Histology Technician'; 'Histonet'; histonet-request at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

We have great luck with Cell Marque antibody on our Ventana Benchmark Ultra using UltraView DAB detection.  He have had dirty staining for spirochetes
(syphilis) in the past utilizing the Red Detection kit and decons do seem to help a bit.  The DAB detection kit is much cleaner.  

-----Original Message-----
From: Sebree Linda A [mailto:LSebree at uwhealth.org]
Sent: Thursday, July 09, 2015 5:16 PM
To: 'Cartun, Richard'; Histology Technician; Histonet; histonet-request at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Rabbit monoclonal, Richard.

Linda A. Sebree
University of Wisconsin Hospital & Clinics IHC/ISH Laboratory, Rm
A4/204-3224
600 Highland Ave. 
Madison, WI 53792
(608)265-6596
FAX: (608)262-7174 

-----Original Message-----
From: Cartun, Richard [mailto:Richard.Cartun at hhchealth.org]
Sent: Thursday, July 09, 2015 4:06 PM
To: Histology Technician; Histonet;
histonet-request at lists.utsouthwestern.edu
Subject: Re: [Histonet] Ventana antibody - H pylori

Is the Ventana antibody polyclonal or monoclonal?

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs Assistant Director, Anatomic Pathology Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 972-1596
(860) 545-2204 Fax

-----Original Message-----
From: Histology Technician [mailto:histology81176 at att.net]
Sent: Thursday, July 09, 2015 4:51 PM
To: Histonet; histonet-request at lists.utsouthwestern.edu
Subject: [Histonet] Ventana antibody - H pylori

 Does anyone else have constant problems with the Ventana antibody H pylori having a dirty background stain?  My pathologists are constantly complaining about it and when I call CS I get the same response...decon the instrument, try a new lot number, etc... it's never truly resolved.  Any thoughts?
Thanks!
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= 

This e-mail message, including any attachments, is for the sole use of the intended recipient(s) and may contain confidential and privileged information. Any unauthorized review, use, disclosure, or distribution is prohibited. If you are not the intended recipient, or an employee or agent responsible for delivering the message to the intended recipient, please contact the sender by reply e-mail and destroy all copies of the original message, including any attachments.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e= 

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.proofpoint.com/v2/url?u=http-3A__lists.utsouthwestern.edu_mailman_listinfo_histonet&d=AwICAg&c=c09Z9T_vWDxmFqobefbEMtlmpncr97OXXiuIfi6sSaw&r=Fh4KlUv_UqKN03sLo_qOgprEKoaSMs5dhn6xDjQ2Oaw&m=RfpXFJ48d3Jhw7L-dEkoNFuBrh2v-_U9uU-J1XmgaI4&s=dItbdub7yicLmpNzAOMuUtJIu1qXu7vhqfR1DsNyAm4&e=